TXNRD3 Protein (AA 1-652) (Strep Tag)

N° du produit ABIN3128044

Détail du produit

Antigène: TXNRD3 (Thioredoxin Reductase 3 (TXNRD3))

Type de proteíne: Recombinant

Attributs du protein: AA 1-652

Origine: Souris

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette TXNRD3 protéine est marqué à la Strep Tag.

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Séquence: MEKPPSPPPP PRAQTSPGLG KVGVLPNRRL GAVRGGLMSS PPGRRARLAS PGTSRPSSEA REELRRRLRD LIEGNRVMIF SKSYCPHSTR VKELFSSLGV VYNILELDQV DDGASVQEVL TEISNQKTVP NIFVNKVHVG GCDRTFQAHQ NGLLQKLLQD DSAHDYDLII IGGGSGGLSC AKEAANLGKK VMVLDFVVPS PQGTTWGLGG TCVNVGCIPK KLMHQAALLG HALQDAKKYG WEYNQQVKHN WEAMTEAIQS HIGSLNWGYR VTLREKGVTY VNSFGEFVDL HKIKATNKKG QETFYTASKF VIATGERPRY LGIQGDKEYC ITSDDLFSLP YCPGCTLVVG ASYVGLECAG FLAGLGLDVT VMVRSVLLRG FDQEMAEKVG SYLEQQGVKF QRKFTPILVQ QLEKGLPGKL KVVAKSTEGP ETVEGIYNTV LLAIGRDSCT RKIGLEKIGV KINEKNGKIP VNDVEQTNVP HVYAIGDILD GKPELTPVAI QAGKLLARRL FGVSLEKCDY INIPTTVFTP LEYGCCGLSE EKAIEMYKKE NLEVYHTLFW PLEWTVAGRD NNTCYAKIIC NKFDNERVVG FHLLGPNAGE ITQGFAAAMK CGLTKQLLDD TIGIHPTCGE VFTTLEITKS SGLDITQKGC UG
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified in one-step affinity chromatography
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (AliCE®).

Pureté: > 80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: TXNRD3 (Thioredoxin Reductase 3 (TXNRD3))

Autre désignation: Txnrd3 (TXNRD3 Produits)

Synonymes: MGC81848 Protein, MGC147163 Protein, TGR Protein, TR2 Protein, TRXR3 Protein, AI196535 Protein, Tgr Protein, thioredoxin reductase 3 Protein, apoptosis-inducing factor 3 Protein, thioredoxin reductase 3 L homeolog Protein, TXNRD3 Protein, LOC413958 Protein, txnrd3.L Protein, txnrd3 Protein, Txnrd3 Protein

Sujet: Thioredoxin reductase 3 (EC 1.8.1.9) (Thioredoxin and glutathione reductase) (Thioredoxin reductase TR2),FUNCTION: Displays thioredoxin reductase, glutaredoxin and glutathione reductase activities. Catalyzes disulfide bond isomerization. Promotes disulfide bond formation between GPX4 and various sperm proteins and may play a role in sperm maturation by promoting formation of sperm structural components. {ECO:0000269|PubMed:11259642, ECO:0000269|PubMed:15901730}.

Poids moléculaire: 71.3 kDa

UniProt: Q99MD6

Pathways: Cell RedoxHomeostasis