MIS12 Protein (AA 1-206) (Strep Tag)

N° du produit ABIN3128636

Détail du produit

Antigène: MIS12 (MIS12, MIND Kinetochore Complex Component, Homolog (MIS12))

Type de proteíne: Recombinant

Attributs du protein: AA 1-206

Origine: Souris

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette MIS12 protéine est marqué à la Strep Tag.

Application: SDS-PAGE (SDS), ELISA, Western Blotting (WB)

Séquence: MSVDPMAYEA QFFGFTPQTC LLRIYVAFQD HLFEVMQAVE QVILKKLEDI PNCEITPVQT RKCTEKFLCF MKGRFDNLFG KMEQLILQSI LCIPPNILLP EDKCQETNPF SEEKLELLQQ EIKELQEKYK VELCTEQALL AELEEQKTVK AKLRETLTFF DELENIGRYQ GTSNFRESLA SLVQSCRKLQ SIRDNVEKES RRLETQ
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: MIS12 (MIS12, MIND Kinetochore Complex Component, Homolog (MIS12))

Autre désignation: Mis12 (MIS12 Produits)

Synonymes: 2510025F08Rik Protein, KNTC2AP Protein, MTW1 Protein, hMis12 Protein, BcDNA:RE19545 Protein, CG18156 Protein, Dmel\\CG18156 Protein, Mis-12 Protein, dmMis12 Protein, AU044651 Protein, Misc12 Protein, RP23-83I13.9 Protein, mtw1 Protein, kntc2ap Protein, MGC80789 Protein, ARABIDOPSIS MINICHROMOSOME INSTABILITY 12 (MIS12)-LIKE Protein, ATMIS12 Protein, MINICHROMOSOME INSTABILITY 12 (MIS12)-LIKE Protein, MINICHROMOSOME INSTABILITY 12 HOMOLOGUE Protein, MIS12 HOMOLOGUE Protein, MOK9.12 Protein, MOK9_12 Protein, MIS12, kinetochore complex component Protein, CG18156 gene product from transcript CG18156-RA Protein, MIS12 kinetochore complex component Protein, MIS12 kinetochore complex component L homeolog Protein, MIS12, MIND kinetochore complex component, homolog (S. pombe) Protein, minichromosome instability 12 (mis12)-like protein Protein, kinetochore protein Mis12 Protein, MIS12 Protein, Mis12 Protein, mis12.L Protein, mis12 Protein

Sujet: Protein MIS12 homolog,FUNCTION: Part of the MIS12 complex which is required for normal chromosome alignment and segregation and for kinetochore formation during mitosis. Essential for proper kinetochore microtubule attachments. {ECO:0000250|UniProtKB:Q9H081}.

Poids moléculaire: 24.1 kDa

UniProt: Q9CY25

Pathways: M Phase