FIP1L1 Protein (AA 1-581) (Strep Tag)

N° du produit ABIN3129277

Détail du produit

Antigène: FIP1L1 (FIP1 Like 1 (FIP1L1))

Type de proteíne: Recombinant

Attributs du protein: AA 1-581

Origine: Souris

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette FIP1L1 protéine est marqué à la Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Séquence: MSAGEVERLV ELSGGTGGDE EEEWLYGGPW DVHVHSDLAK DLDENEVERP EEENASANPP SGIEEEAAEN GVAKPKVTET EDDSDSDSDD DEDDVHVTIG DIKTGAPQYG SYGTAPVNLN IKAGGRVYGN TGTKVKGVDL DAPGSINGVP LLEVDLDSFE DKPWRKPGAD LSDYFNYGFN EDTWKAYCEK QKRIRMGLEV IPVTSTTNKI TVQQGRTGNS EKEAALPSTK AEFTSPPSLF KTGLPPSRNS TSSQSQTSTA SRKASSSVGK WQDRYGRAES PDLRRLPGAI DVIGQTITIS RVEGRRRANE NSNIQVLSDR SATEVDNNFS KPPPFFPPGA PPTHLPPPPF LPPPPTVSTA PPLIPPPGIP ITVPPPGFPP PPGAPPPSLI PTIESGHSSG YDSRSARAFP YGNVAFPHLT SSAPSWPSLV DTTKQWDYYA RREKDRDRDR ERDRDRERER DRDRERERTR ERERERDHSP TPSVFNSDEE RYRYREYAER GYERHRASRE KEERHRERRH REKEETRHKS SRSNSRRRHE SEEGDSHRRH KHKKSKRSKE GKEAGSEPVP EQESTEAAPA E
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: FIP1L1 (FIP1 Like 1 (FIP1L1))

Autre désignation: Fip1l1 (FIP1L1 Produits)

Synonymes: FIP1 Protein, Rhe Protein, hFip1 Protein, 1300019H17Rik Protein, Rje Protein, fip1l1 Protein, wu:fd36f11 Protein, zgc:103421 Protein, factor interacting with PAPOLA and CPSF1 Protein, factor interacting with PAPOLA and CPSF1 S homeolog Protein, FIP1 like 1 (S. cerevisiae) Protein, FIP1 like 1b (S. cerevisiae) Protein, fip1l1 Protein, FIP1L1 Protein, fip1l1.S Protein, Fip1l1 Protein, fip1l1b Protein

Sujet: Pre-mRNA 3'-end-processing factor FIP1 (FIP1-like 1 protein),FUNCTION: Component of the cleavage and polyadenylation specificity factor (CPSF) complex that plays a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. FIP1L1 contributes to poly(A) site recognition and stimulates poly(A) addition. Binds to U-rich RNA sequence elements surrounding the poly(A) site. May act to tether poly(A) polymerase to the CPSF complex (By similarity). {ECO:0000250}.

Poids moléculaire: 65.0 kDa

UniProt: Q9D824