Ifi202b Protein (AA 1-445) (Strep Tag)

N° du produit ABIN3130641

Détail du produit

Antigène: Ifi202b (Interferon Activated Gene 202B (Ifi202b))

Type de proteíne: Recombinant

Attributs du protein: AA 1-445

Origine: Souris

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette Ifi202b protéine est marqué à la Strep Tag.

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Séquence: MSNRNLRSST NSEFSEGQHQ TPSSDSSGHG EDQPQASPGP NKKSHTPKKN ISKGAVLHEK PMTVMVLTAT EPFNYKEGKE NMFHATVATE SQYYRVKVFN MDLKEKFTEN KFITISKYFN SSGILEINET ATVSEAAPNQ IIEVPKNIIR SAKETLKISK IKELDSGTLI YGVFAVEKKK VNDKSITFKI KDNEDNIKVV WDKKQHNINY EKGDKLQLFS FHLRKGNGKP ILHSGNHSFI KGEKLLKESF EGDGYHKGPK QVVALKATKL FTYDSIKSKK MFHATVATDT EFFRVMVFEE NLEKKFIPGN TIALSDYFGM YGSLAIHEYS SVSEVKSQNK EDSSSSDERL IEHLKICDLH LQTKERLVDG EFKVYRKSTG NNCICYGIWD DTGAMKVVVS GQLTSVNCEI GNTIRLVCFE LTSNADEWFL RSTRYSYMEV IMPEK
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: Ifi202b (Interferon Activated Gene 202B (Ifi202b))

Autre désignation: Ifi202 (Ifi202b Produits)

Synonymes: Ifbip-1 Protein, Ifi202 Protein, Ifi202a Protein, p202 Protein, interferon activated gene 202B Protein, Ifi202b Protein

Sujet: Interferon-activable protein 202 (Ifi-202) (Interferon-inducible protein p202) (Lupus susceptibility protein p202),FUNCTION: DNA-binding protein involved in innate immune response and has anti-inflammatory activity (PubMed:19131592, PubMed:23850291, PubMed:23567559). Inhibits caspase activation in response to cytosolic DNA by preventing activation of the AIM2 inflammasome, probably by sequestering cytoplasmic DNA and preventing its being bound by AIM2 (PubMed:19131592, PubMed:23850291, PubMed:23567559). Also inhibits activation of the AIM2 inflammasome via a direct interaction with AIM2, which prevents the interaction between AIM2 and PYCARD and formation of the AIM2 inflammasome (PubMed:23850291). Binds double-stranded DNA (dsDNA) in the cytosol (PubMed:19131592, PubMed:23850291, PubMed:23567559, PubMed:24419611). Has anti-apoptotic effects due to inhibition of the transcriptional activity of TP53/p53 (PubMed:16670293). Inhibits the transcriptional activity of several transcription factors, including NF-kappa-B p50 and p65, FOS, JUN, E2F1, E2F4, MYOD1 and myogenin (PubMed:8524315). {ECO:0000269|PubMed:16670293, ECO:0000269|PubMed:19131592, ECO:0000269|PubMed:23567559, ECO:0000269|PubMed:23850291, ECO:0000269|PubMed:24419611, ECO:0000269|PubMed:8524315}.

Poids moléculaire: 50.5 kDa

UniProt: Q9R002