CCDC147 Protein (AA 1-873) (Strep Tag)

N° du produit ABIN3131026

Détail du produit

Antigène: CCDC147 (Coiled-Coil Domain Containing 147 (CCDC147))

Type de proteíne: Recombinant

Attributs du protein: AA 1-873

Origine: Souris

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette CCDC147 protéine est marqué à la Strep Tag.

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Séquence: MTEDKAEKVM PEETAFEEIE KDFQEVLSEL SGDKSLEKFR TEYEKLHSIM KKSYENEKRL MAKCRELNAE IVVNSAKVAT ALKLSQDDQT TIASLKKEIE KAWKMVDSAY DKEQKAKETI LALKEEIVNL TKLVEQGSGL SMDQDSNIRD LLKFKEEVTK ERDQLLSEVV KLRENLAQTI EKQQAAEHAK EEAEMAISQF QQEIQHRQNE ASRESRKKEK LEKELRQIQT DMDGRQAEIK AMQQYMHKSK EELQRLEQQL KEQKILNERA AKEVEQFQMR NAKLQQENDQ HTLTCEQLSQ ENQQKALELK AKEDEIHQMR LDLGKLNKIR EQIHKKLHQL DDQKAEVEQQ KDTLKNQILG LEREVESSKK QAELDKKAME ELLRERDILN KNMLKAVSAT QKQVDLVKLH EQAKKNLEEE IQNYKDEAQK QRKIIFQLEK ERDRYINEAS DLTQRVLANM EDIKVREIQI FDYRKKIAES ETKLKQQQNL YEAVRSDRNL YSKNLVEAQD EITEMKRKLK IMTHQVDQLK EEISAKEAAL VKLHLEQQRI EKEKETLKAE LQKLRQQALE TKHFIEKQEV EERKLLRIIA EADGERVRQK KELDQVISER DILGSQLVRR NDELALLYEK IKIQQSVLNK GETQYNQRVE DMRILKLEIK KLRREKGILA RSVANVEELR QELYHMQREF LKERTRCRAL EEELENPMNV HRWRKLEASD PSTFELIQKI HTLQKRLISK TEEVVEKELL LQEKEKLYVE LKHILARQPG PEAAEQLQIY RHTLREKTKQ LKVLSSELNM YESQSQEYKY EIERLGNELM SLKKKYLAQK RKELVLKNKD RMSMNNIFSE TKKSVPRFTG GGFPLHQATK VKF
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: CCDC147 (Coiled-Coil Domain Containing 147 (CCDC147))

Autre désignation: Cfap58 (CCDC147 Produits)

Synonymes: Gm969 Protein, RGD1307961 Protein, zgc:153620 Protein, C10orf80 Protein, bA127L20.4 Protein, bA127L20.5 Protein, bA554P13.1 Protein, cilia and flagella associated protein 58 Protein, coiled-coil domain containing 147 Protein, cilia- and flagella-associated protein 58 Protein, Cfap58 Protein, Ccdc147 Protein, CFAP58 Protein, cfap58 Protein, LOC100187286 Protein, LOC100206293 Protein

Sujet: Cilia- and flagella-associated protein 58,FUNCTION: Has an essential role in the assembly and organization of the sperm flagellar axoneme (PubMed:32791035). Required for the elongation of the primary cilium and sperm flagellar midpiece via modulation of the Notch signaling pathway (PubMed:31904090). {ECO:0000269|PubMed:31904090, ECO:0000269|PubMed:32791035}.

Poids moléculaire: 103.5 kDa

UniProt: B2RW38