Plexin B2 Protein (PLXNB2) (AA 1223-1842) (His tag)

N° du produit ABIN3131039

Détail du produit

Antigène: Plexin B2 (PLXNB2)

Type de proteíne: Recombinant

Attributs du protein: AA 1223-1842

Origine: Souris

Source: Cellules d'insectes

Purification/Conjugué: Cette Plexin B2 protéine est marqué à la His tag.

Application: ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)

Séquence: CYWRKSQQAE REYEKIKSQL EGLEESVRDR CKKEFTDLMI EMEDQTNDVH EAGIPTLDYK TYTDRVFFLP SKDGDKDVMI TGKLDIPESR RPIVEQALYQ FSNLLNSKSF LINFIHTLEN QREFSARAKV YFASLLTVAL HGKLEYYTDI MRTLFLELME QYVVAKNPKL MLRRSETVVE RMLSNWMSIC LYQYLKDSAG EPLYKLFKAI KHQVEKGPVD AVQKKAKYTL NDTGLLGDDV EYAPLTVSVI VQDEGIDAIP VKVLNCDTIS QVKEKIIDQV YRTQPCSCWP KPDSVVLEWR PGSTAQILSD LDLTSQREGR WKRINTLMHY NVRDGATLIL SKVGVSQQPE DSQQDLPGER HALLEEENRV WHLVRPTDEV DEGKSKRGSM KEKERTKAIT EIYLTRLLSV KGTLQQFVDN FFQSVLAPGH AVPPAVKYFF DFLDEQAEKH DIRDEDTIHI WKTNSLPLRF WVNILKNPHF IFDVHVHEVV DASLSVIAQT FMDACTRTEH KLSRDSPSNK LLYAKEISTY KKMVEDYYKG IRQMVQVSDQ DMNTHLAEIS RAHTDSLNTL VALHQLYQYT QKYYDEIINA LEEDPAAQKM QLAFRLQQIA AALENKVTDL
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Plxnb2 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Commentaires:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: Plexin B2 (PLXNB2)

Autre désignation: Plxnb2 (PLXNB2 Produits)

Synonymes: PLXNB2 Protein, MM1 Protein, Nbla00445 Protein, PLEXB2 Protein, dJ402G11.3 Protein, 1110007H23Rik Protein, Debt Protein, mKIAA0315 Protein, plexin B2 Protein, plexin B2 S homeolog Protein, PLXNB2 Protein, plxnb2.S Protein, plxnb2 Protein, Plxnb2 Protein

Sujet: Cell surface receptor for SEMA4C, SEMA4D and SEMA4G that plays an important role in cell-cell signaling. Binding to class 4 semaphorins promotes downstream activation of RHOA and phosphorylation of ERBB2 at 'Tyr-1248'. Required for normal differentiation and migration of neuronal cells during brain corticogenesis and for normal embryonic brain development. Regulates the migration of cerebellar granule cells in the developing brain. Plays a role in RHOA activation and subsequent changes of the actin cytoskeleton. Plays a role in axon guidance, invasive growth and cell migration. May modulate the activity of RAC1 and CDC42. Down-regulates macrophage migration in wound-healing assays (in vitro). {ECO:0000269|PubMed:17554007, ECO:0000269|PubMed:19948886, ECO:0000269|PubMed:21122816, ECO:0000269|PubMed:21966369}.

Poids moléculaire: 72.8 kDa Including tag.

UniProt: B2RXS4

Pathways: Tube Formation