SDCBP Protein (AA 2-299) (His tag)

N° du produit ABIN3131359

Détail du produit

Antigène: SDCBP (Syndecan Binding Protein (Syntenin) (SDCBP))

Type de proteíne: Recombinant

Attributs du protein: AA 2-299

Origine: Souris

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette SDCBP protéine est marqué à la His tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Séquence: SLYPSLEDLK VDKVIQAQTA YSANPASQAF VLVDASAALP PDGNLYPKLY PELSQYMGLS LNEAEICESM PMVSGAPAQG QLVARPSSVN YMVAPVTGND AGIRRAEIKQ GIREVILCKD QDGKIGLRLK SIDNGIFVQL VQANSPASLV GLRFGDQVLQ INGENCAGWS SDKAHKVLKQ AFGEKITMTI RDRPFERTVT MHKDSSGHVG FIFKSGKITS IVKDSSAARN GLLTDHHICE INGQNVIGLK DAQIADILST AGTVVTITIM PTFIFEHIIK RMAPSIMKSL MDHTIPEV
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Sdcbp Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in bacterial culture:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Endotoxin has not been removed. Please contact us if you require endotoxin removal.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Commentaires:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: SDCBP (Syndecan Binding Protein (Syntenin) (SDCBP))

Autre désignation: Sdcbp (SDCBP Produits)

Synonymes: MDA-9 Protein, ST1 Protein, SYCL Protein, TACIP18 Protein, Sycl Protein, syntenin-1 Protein, mda-9 Protein, st1 Protein, sycl Protein, tacip18 Protein, MGC81274 Protein, syntenin Protein, wu:fc51c03 Protein, wu:fk31e01 Protein, zgc:55679 Protein, zgc:77716 Protein, sb:eu862 Protein, si:dkey-235k4.1 Protein, syndecan binding protein Protein, syndecan binding protein L homeolog Protein, syndecan binding protein (syntenin) 2 Protein, syndecan binding protein (syntenin) Protein, SDCBP Protein, Sdcbp Protein, sdcbp.L Protein, LOC732879 Protein, sdcbp2 Protein, sdcbp Protein

Sujet: Multifunctional adapter protein involved in diverse array of functions including trafficking of transmembrane proteins, neuro and immunomodulation, exosome biogenesis, and tumorigenesis. Positively regulates TGFB1-mediated SMAD2/3 activation and TGFB1-induced epithelial-to-mesenchymal transition (EMT) and cell migration in various cell types. May increase TGFB1 signaling by enhancing cell-surface expression of TGFR1 by preventing the interaction between TGFR1 and CAV1 and subsequent CAV1-dependent internalization and degradation of TGFR1. In concert with SDC1/4 and PDCD6IP, regulates exosome biogenesis (By similarity). Regulates migration, growth, proliferation, and cell cycle progression in a variety of cancer types (PubMed:26539120). In adherens junctions may function to couple syndecans to cytoskeletal proteins or signaling components. Seems to couple transcription factor SOX4 to the IL-5 receptor (IL5RA). May also play a role in vesicular trafficking. Seems to be required for the targeting of TGFA to the cell surface in the early secretory pathway (By similarity). {ECO:0000250|UniProtKB:O00560, ECO:0000269|PubMed:26539120}.

Poids moléculaire: 33.2 kDa Including tag.

UniProt: O08992