AP3D1 Protein (AA 2-1199) (His tag)

N° du produit ABIN3131746

Détail du produit

Antigène: AP3D1 (Adaptor-Related Protein Complex 3, delta 1 Subunit (AP3D1))

Type de proteíne: Recombinant

Attributs du protein: AA 2-1199

Origine: Souris

Source: Cellules d'insectes

Purification/Conjugué: Cette AP3D1 protéine est marqué à la His tag.

Application: Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Séquence: ALKMVKGSID RMFDKNLQDL VRGIRNHKED EAKYISQCID EIKQELKQDN IAVKANAVCK LTYLQMLGYD ISWAAFNIIE VMSASKFTFK RVGYLAASQC FHEGTDVIML TTNQIRKDLS SPSQYDTGVA LTGLSCFVTP DLARDLANDI MTLMSHTKPY IRKKAVLIMY KVFLKYPESL RPAFPRLKEK LEDPDPGVQS AAVNVICELA RRNPKNYLSL APLFFKLMTS STNNWVLIKI IKLFGALTPL EPRLGKKLIE PLTNLIHSTS AMSLLYECVN TVIAVLISLS SGMPNHSASI QLCVQKLRIL IEDSDQNLKY LGLLAMSKIL KTHPKSVQSH KDLILQCLDD KDESIRLRAL DLLYGMVSKK NLMEIVKKLM THVDKAEGTT YRDELLTKII DICSQSNYQH ITNFEWYISI LVELTRLEGT RHGHLIAAQM LDVAIRVKAI RKFAVSQMSS LLDSAHLVAS STQRNGICEV LYAAAWICGE FSEHLQGPQQ TLEAMLRPKV TTLPGHIQAV YVQNVVKLYA SILQQKEQAA DTEAAQEVTQ LLVERLPQFV QSADLEVQER ASCILQLVKH VQKLQAKGVP VAEEVSALFA GELNPVAPKA QKKVPVPEGL DLDAWINEPP SDSESEDEKP KAIFHEEEPR HTRRRQPEED EEELARRREA RKQEQANNPF YIKSSPSPQK RYQDAPGVEH IPVVQIDLSV PLKVPGMPMS DQYVKLEEQR RHRQRLEKDK KRKKKEKGKR RHSSLPTESD EDIAPAQRVD IITEEMPENA LPSDEDDKDP NDPYRALDID LDKPLADSEK LPVQKHRNAE AVKSPEKEGV LGVEKKSKKP KKKEKKTKER EREKKDKKGE DLDFWLSTTP PPAAAPIPAP STEELAASTI TSPKDECEVL KGEEEDHVDH DQERKSSRHK KKKHRKEKEK EERPRDKKKA KKKQVAPLEN GAAAEEEEEP IPPMSSYCLL AESPYIKVTY DIQASLQKDS QVTVSIILEN QSSSFLKNME LNVLDSLNTK MTRPEGSSVH DGVPVPFQLP PGVSNEAQFV FTIQSIVMAQ KLKGTLSFIA KDDEGATHEK LDFRLHFSCS SYLITTPCYS DAFAKLLESG DLSMNSIKVD GISMSFQNLL AKICFYHHFS VVERVDSCAS MYSRSIQGHH VCLLVKKGES SVSVDGKCSD ATLLSSLLEE MKTTLAQC
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Ap3d1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Commentaires:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: AP3D1 (Adaptor-Related Protein Complex 3, delta 1 Subunit (AP3D1))

Autre désignation: Ap3d1 (AP3D1 Produits)

Synonymes: wu:fj51g01 Protein, si:ch211-129c21.6 Protein, ADTD Protein, hBLVR Protein, AA407035 Protein, Ap3d Protein, Bolvr Protein, mBLVR1 Protein, mh Protein, mocha Protein, BLVR Protein, adaptor-related protein complex 3, delta 1 subunit Protein, AP-3 complex subunit delta-1 Protein, adaptor related protein complex 3 delta 1 subunit Protein, ap3d1 Protein, EDI_172030 Protein, EHI_158840 Protein, Tsp_04773 Protein, AP3D1 Protein, Ap3d1 Protein

Sujet: Part of the AP-3 complex, an adaptor-related complex which is not clathrin-associated. The complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to lysosomes (By similarity). In concert with the BLOC-1 complex, AP-3 is required to target cargos into vesicles assembled at cell bodies for delivery into neurites and nerve terminals. {ECO:0000250, ECO:0000269|PubMed:21998198}.

Poids moléculaire: 135.9 kDa Including tag.

UniProt: O54774