SEMA3F Protein (AA 19-785) (His tag)

N° du produit ABIN3132171

Détail du produit

Antigène: SEMA3F (Sema Domain, Immunoglobulin Domain (Ig), Short Basic Domain, Secreted, (Semaphorin) 3F (SEMA3F))

Type de proteíne: Recombinant

Attributs du protein: AA 19-785

Origine: Souris

Source: Cellules d'insectes

Purification/Conjugué: Cette SEMA3F protéine est marqué à la His tag.

Application: SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)

Séquence: ATPIQDQLPA TPRVRLSFKE LKATGTAHFF NFLLNTTDYR ILLKDEDHDR MYVGSKDYVL SLDLHDINRE PLIIHWAASP QRIEECILSG KDGNGECGNF VRLIQPWNRT HLYVCGTGAY NPMCTYVNRG RRAQALPWTQ MQVVRGRGSR ATDGADRPTP TAPRQDYIFY LEPEKLESGK GKCPYDPKLD TASALINEEL YAGVYIDFMG TDAAIFRTLG KQTAMRTDQY NSRWLNDPSF IHAELIPDSA ERNDDKLYFF FRERSAEAPQ NPAVYARIGR ICLNDDGGHC CLVNKWSTFL KARLVCSVPG EDGIETHFDE LQDVFVQQTQ DVRNPVIYAV FTSSGSVFRG SAVCVYSMAD IRMVFNGPFA HKEGPNYQWM PFSGKMPYPR PGTCPGGTFT PSMKSTKDYP DEVINFMRTH PLMYQAVYPL QRRPLVVRTG APYRLTTVAV DQVDAADGRY EVLFLGTDRG TVQKVIVLPK DDQEVEELML EEVEVFKEPA PVKTMTISSK RQQLYVASAV GVTHLSLHRC QAYGAACADC CLARDPYCAW DGQACSRYTA SSKRRSRRQD VRHGNPIRQC RGFNSNANKN AVESVQYGVA GSAAFLECQP RSPQATVKWL FQRDPSDRRR EIRAEDRFLR TEQGLLLRAL QLGDRGLYSC TATENNFKHI VTRVQLHVLG RDAVHAALFP PLAVSVPPPP GTGPPTPPYQ ELAQLLAQPE VGLIHQYCQG YWRHVPPRPR EAPGALRPPE LQDQKKPRNR RHHPPDT
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Sema3f Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Commentaires:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: SEMA3F (Sema Domain, Immunoglobulin Domain (Ig), Short Basic Domain, Secreted, (Semaphorin) 3F (SEMA3F))

Autre désignation: Sema3f (SEMA3F Produits)

Synonymes: Sema4 Protein, Semak Protein, SemaIV Protein, semaphorin-3F Protein, sema4 Protein, semak Protein, sema-iv Protein, SEMA3F Protein, SEMA-IV Protein, SEMA4 Protein, SEMAK Protein, sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3F Protein, semaphorin 3F Protein, semaphorin 3F L homeolog Protein, Sema3f Protein, SEMA3F Protein, sema3f Protein, sema3f.L Protein

Poids moléculaire: 87.5 kDa Including tag.

UniProt: O88632

Pathways: Regulation of Cell Size