NR2F6 Protein (AA 1-390) (Strep Tag)

N° du produit ABIN3134056

Détail du produit

Antigène: NR2F6 (Nuclear Receptor Subfamily 2, Group F, Member 6 (NR2F6))

Type de proteíne: Recombinant

Attributs du protein: AA 1-390

Origine: Souris

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette NR2F6 protéine est marqué à la Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Séquence: MAMVTGGWGD PGGDTNGVDK AGGSYPRATE DDSASPPGAT SDAEPGDEER PGLQVDCVVC GDKSSGKHYG VFTCEGCKSF FKRSIRRNLS YTCRSNRDCQ IDQHHRNQCQ YCRLKKCFRV GMRKEAVQRG RIPHALPGPA ACSPPGATGV EPFTGPPVSE LIAQLLRAEP YPAAGRFGGG GAVLGIDNVC ELAARLLFST VEWARHAPFF PELPAADQVA LLRLSWSELF VLNAAQAALP LHTAPLLAAA GLHAAPMAAE RAVAFMDQVR AFQEQVDKLG RLQVDAAEYG CLKAIALFTP DACGLSDPAH VESLQEKAQV ALTEYVRAQY PSQPQRFGRL LLRLPALRAV PASLISQLFF MRLVGKTPIE TLIRDMLLSG STFNWPYGSG
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: NR2F6 (Nuclear Receptor Subfamily 2, Group F, Member 6 (NR2F6))

Autre désignation: Nr2f6 (NR2F6 Produits)

Synonymes: EAR-2 Protein, EAR2 Protein, ERBAL2 Protein, AV090102 Protein, COUP-TF3 Protein, Erbal2 Protein, COUP(II) Protein, NR2F6 Protein, couptf2 Protein, fc94g11 Protein, wu:fc94g11 Protein, zgc:77259 Protein, ear-2 Protein, ear2 Protein, erbal2 Protein, nr2f6l Protein, wu:fc72d04 Protein, zgc:77260 Protein, nuclear receptor subfamily 2 group F member 6 Protein, nuclear receptor subfamily 2, group F, member 6 Protein, nuclear receptor subfamily 2, group F, member 6a Protein, nuclear receptor subfamily 2 group F member 6 L homeolog Protein, nuclear receptor subfamily 2, group F, member 6b Protein, NR2F6 Protein, Nr2f6 Protein, nr2f6a Protein, nr2f6.L Protein, nr2f6 Protein, nr2f6b Protein

Sujet: Nuclear receptor subfamily 2 group F member 6 (COUP transcription factor 3) (COUP-TF3) (V-erbA-related protein 2) (EAR-2),FUNCTION: Transcription factor predominantly involved in transcriptional repression. Binds to promoter/enhancer response elements that contain the imperfect 5'-AGGTCA-3' direct or inverted repeats with various spacings which are also recognized by other nuclear hormone receptors. Involved in modulation of hormonal responses. Represses transcriptional activity of the lutropin-choriogonadotropic hormone receptor/LHCGR gene, the renin/REN gene and the oxytocin-neurophysin/OXT gene. Represses the triiodothyronine-dependent and -independent transcriptional activity of the thyroid hormone receptor gene in a cell type-specific manner. The corepressing function towards thyroid hormone receptor beta/THRB involves at least in part the inhibition of THRB binding to triiodothyronine response elements (TREs) by NR2F6. Inhibits NFATC transcription factor DNA binding and subsequently its transcriptional activity. Acts as transcriptional repressor of IL-17 expression in Th-17 differentiated CD4(+) T cells and may be involved in induction and/or maintenance of peripheral immunological tolerance and autoimmunity. Involved in development of forebrain circadian clock, is required early in the development of the locus coeruleus (LC). {ECO:0000269|PubMed:12690040, ECO:0000269|PubMed:15741322, ECO:0000269|PubMed:18701084}.

Poids moléculaire: 42.0 kDa

UniProt: P43136

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Photoperiodism