RNF216 Protein (AA 1-853) (Strep Tag)

N° du produit ABIN3134535

Détail du produit

Antigène: RNF216 (Ring Finger Protein 216 (RNF216))

Type de proteíne: Recombinant

Attributs du protein: AA 1-853

Origine: Souris

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette RNF216 protéine est marqué à la Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Séquence: MAEGNNKEEV IHLNNFPCHR GKEWMAVREG PITISDSSDE EGIPMLVTPA TEQQEDDLDD DVILTEDDSE DEYGGFLDLE SGKKEGEAKP GPSSKQTADD IVNPRLEQKV IILGENGLLF PESEPLEVQN QSSEDSETEL LSNPGEPAAS VDDQLIGEEY WLDHPYFQAP NPQPQERTNQ VVPQERHSES EMGPMFFRHD FPEPAFPRPE PQQEGIPGPA SPQPAHPLGE LEDQQLAIDE DPGPAFPLSG PQEANLANMW EQEAAEVDQD LIPLLVKETE ARFPDVASGY VEEIIHLKNY YDLNVLCNFL LENPDYPKRE DRLIIHPSSS LLASQDDAKL PKIDFFDYSK LTPLDQRCFI QAADLLMADF KMLSSQDIKW ALHELKGHYA ITRKAFSDAI KKWQELSPET SGKRKKRKEM NQYSFIDFKF EQGNIKIEKR MFFLENKRRH CRYYDHQALL PAVKQEQEFY EQKIKEMAEH EDFLLALQMN EEQYQKDGQL IECRCCYGEF PFEELTQCAD AHLFCKECLI RYAQEAVFGS GKSELSCMEG SCTCSFPTSE LEKVLPQTIL YKYYERKAEE EVAAAYADEL VRCPSCSFPA LLDSDVKRFS CPNPRCRKET CRKCQGLWKE HNGLTCEELA EKDDIKYRTS IEEKMTAARI RKCHKCGTGL IKSEGCNRMS CRCGAQMCYL CRVSINGYDH FCQHPRSPGA PCQECSRCSL WTDPTEDDEK LIEEIQKEAE EEQKRKNGEN TFKRIGPPLE KPAEKVQRVE ALPRPVPQNL HPQMPPYAFV HPPFPLPPVR PVFNNFPINM GPVPAPYVPP LPNVRVNYDF GHMHVPLEHN LPMHFGPQPR HRF
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: RNF216 (Ring Finger Protein 216 (RNF216))

Autre désignation: Rnf216 (RNF216 Produits)

Synonymes: RGD1565135 Protein, TRIAD3 Protein, RNF216 Protein, zin Protein, u7i1 Protein, triad3 Protein, ubce7ip1 Protein, MGC147026 Protein, 2810055g22rik Protein, 2810055G22Rik Protein, AI647468 Protein, AU019462 Protein, C86502 Protein, F830018F18Rik Protein, UIP83 Protein, Ubce7ip1 Protein, CAHH Protein, U7I1 Protein, UBCE7IP1 Protein, ZIN Protein, ring finger protein 216 Protein, Rnf216 Protein, RNF216 Protein, rnf216 Protein

Sujet: E3 ubiquitin-protein ligase RNF216 (EC 2.3.2.27) (RING finger protein 216) (RING-type E3 ubiquitin transferase RNF216) (Triad domain-containing protein 3) (UbcM4-interacting protein 83) (Ubiquitin-conjugating enzyme 7-interacting protein 1),FUNCTION: E3 ubiquitin ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their ubiquitination. Plays a role in the regulation of antiviral responses by promoting the degradation of TRAF3, TLR4 and TLR9. In turn, down-regulates NF-kappa-B and IRF3 activation as well as beta interferon production. Participates also in the regulation of autophagy by ubiquitinating BECN1 leading to its degradation and autophagy inhibition. Plays a role in ARC-dependent synaptic plasticity by mediating ARC ubiquitination resulting in its rapid proteasomal degradation (By similarity). Plays aso an essential role in spermatogenesis and male fertility (PubMed:30649198). Mechanistically, regulates meiosis by promoting the degradation of PRKACB through the ubiquitin-mediated lysosome pathway (PubMed:33724554). Modulates the gonadotropin-releasing hormone signal pathway by affecting the stability of STAU2 that is required for the microtubule-dependent transport of neuronal RNA from the cell body to the dendrite (PubMed:37439148). {ECO:0000250|UniProtKB:Q9NWF9, ECO:0000269|PubMed:30649198, ECO:0000269|PubMed:33724554, ECO:0000269|PubMed:37439148}.

Poids moléculaire: 97.7 kDa

UniProt: P58283