EIF3E Protein (AA 2-445) (His tag)

N° du produit ABIN3134567

Détail du produit

Antigène: EIF3E (Eukaryotic Translation Initiation Factor 3 Subunit E (EIF3E))

Type de proteíne: Recombinant

Attributs du protein: AA 2-445

Origine: Souris

Source: Cellules d'insectes

Purification/Conjugué: Cette EIF3E protéine est marqué à la His tag.

Application: ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)

Séquence: AEYDLTTRIA HFLDRHLVFP LLEFLSVKEI YNEKELLQGK LDLLSDTNMV DFAMDVYKNL YSDDIPHALR EKRTTVVAQL KQLQAETEPI VKMFEDPETT RQMQSTRDGR MLFDYLADKH GFRQEYLDTL YRYAKFQYEC GNYSGAAEYL YFFRVLVPAT DRNALSSLWG KLASEILMQN WDAAMEDLTR LKETIDNNSV SSPLQSLQQR TWLIHWSLFV FFNHPKGRDN IIDLFLYQPQ YLNAIQTMCP HILRYLTTAV ITNKDVRKRR QVLKDLVKVI QQESYTYKDP ITEFVECLYV NFDFDGAQKK LRECESVLVN DFFLVACLED FIENARLFIF ETFCRIHQCI SINMLADKLN MTPEEAERWI VNLIRNARLD AKIDSKLGHV VMGNNAVSPY QQVIEKTKSL SFRSQMLAMN IEKKLNQNSR SEAPNWATQD SGFY
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Eif3e Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Commentaires:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: EIF3E (Eukaryotic Translation Initiation Factor 3 Subunit E (EIF3E))

Autre désignation: Eif3e (EIF3E Produits)

Synonymes: EIF3-P48 Protein, EIF3S6 Protein, INT6 Protein, eIF3-p46 Protein, 48kDa Protein, Eif3s6 Protein, Int6 Protein, eIF3-p48 Protein, eif3-p48 Protein, eif3s6 Protein, eif3s6-A Protein, eIF3e Protein, eIF3-S6 Protein, eif3s6-B Protein, EIF3SE Protein, eif3s6b Protein, im:7147439 Protein, QtsA-18056 Protein, eukaryotic translation initiation factor 3 subunit E Protein, eukaryotic translation initiation factor 3, subunit E Protein, eukaryotic translation initiation factor 3 subunit E L homeolog Protein, eukaryotic translation initiation factor 3 subunit 6 Protein, eukaryotic translation initiation factor 3 subunit E S homeolog Protein, eukaryotic translation initiation factor 3, subunit E, a Protein, Eukaryotic translation initiation factor 3 subunit E Protein, eukaryotic translation initiation factor 3, subunit E, b Protein, EIF3E Protein, Eif3e Protein, eif3e Protein, eif3e.L Protein, LOC692936 Protein, eif3e.S Protein, eif3ea Protein, eif-3.E Protein, eif3eb Protein, LOC108348260 Protein, int6 Protein

Sujet: Required for nonsense-mediated mRNA decay (NMD), may act in conjunction with UPF2 to divert mRNAs from translation to the NMD pathway. May interact with MCM7 and EPAS1 and regulate the proteasome-mediated degradation of these proteins (By similarity). Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. {ECO:0000250}.

Poids moléculaire: 53.0 kDa Including tag.

UniProt: P60229

Pathways: Ribonucleoprotein Complex Subunit Organization, Hepatitis C