FCHO2 Protein (AA 1-809) (Strep Tag)

N° du produit ABIN3135079

Détail du produit

Antigène: FCHO2 (FCH Domain Only 2 (FCHO2))

Type de proteíne: Recombinant

Attributs du protein: AA 1-809

Origine: Souris

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette FCHO2 protéine est marqué à la Strep Tag.

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Séquence: MVMAHFVENF WGEKNNGFDV LYHNMKHGQI STKELADFVR ERATIEEAYS RSMTKLAKSA SNYSQLGTFA PMWDVFKTST EKLANCHLDL VRKLQELIKE VQKYGEEQVK SHKKTKEEVA GTLEAVQAIQ NITQALQKSK ENYTAKCVEQ ERLKKEGATQ REIEKAAVKS KKATDTYKLY VEKYALTKAD FEQKMTETAQ KFQDIEETHL IHIKEIIGSL SNAVKEIHLQ IGQVHEEFIN NMANTTIESL IQKFAESKGT GKERPGLIEF EECDPASAVE GIKPRKRKTF ALPGIIKKEK DAESVECPDA DSLNIPDVDE EGFSIKPEAN QNDTKENHFY SSSDSDSEDE EPKRYRIEIK PAHPNNLHHT MASLDELKVS IGNITLSPAV SRHSPVQMNR NSSNEELTKS KPSSLPTEKG TNDLLAWDPL FGSSLESSSA PLTSSSSARP TTPLSLGTLV PPPRPASRPK LASGKLSGIN EIPRPFSPPV TSNTSPPPTA PLARAESSSS ISSSASLSAA NTPTVGVSRG PSPVSLGNQD TLPVAIALTE SVNAYFKGAD PTKCIVKITG DVTISFPSGI IKVFTSNPSP AVLCFRVKNI SRLEQILPNS QLVFSDPSQC DSNTKDFWMN MQAVTIYLKK LSEQNPAASY YNVDVLKYQV SSNGIQSTPL NLATYWKCSA STTDLRVDYK YNPEAMVAPS VLSNIQVVVP VDGGVTNMQS LPPAIWNAEQ MKAFWKLSGI SEKSDSGGSG SLRAKFDLSE GPSKPTTLAV QFLSEGNTLS GVDIELVGTG YRLSLVKKRF ATGRYLADC
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: FCHO2 (FCH Domain Only 2 (FCHO2))

Autre désignation: Fcho2 (FCHO2 Produits)

Synonymes: 5832424M12Rik Protein, AA387320 Protein, RGD1565396 Protein, cb304 Protein, sb:cb304 Protein, wu:fa01a02 Protein, zgc:112167 Protein, FCH domain only 2 Protein, FCHO2 Protein, fcho2 Protein, Fcho2 Protein

Sujet: F-BAR domain only protein 2,FUNCTION: Functions in an early step of clathrin-mediated endocytosis. Has both a membrane binding/bending activity and the ability to recruit proteins essential to the formation of functional clathrin-coated pits. Has a lipid-binding activity with a preference for membranes enriched in phosphatidylserine and phosphoinositides (Pi(4,5) biphosphate) like the plasma membrane. Its membrane-bending activity might be important for the subsequent action of clathrin and adaptors in the formation of clathrin-coated vesicles. Involved in adaptor protein complex AP-2-dependent endocytosis of the transferrin receptor, it also functions in the AP-2-independent endocytosis of the LDL receptor. {ECO:0000269|PubMed:21762413, ECO:0000269|PubMed:21883765}.

Poids moléculaire: 88.7 kDa

UniProt: Q3UQN2