PFAS Protein (AA 1-1337) (Strep Tag)

N° du produit ABIN3135228

Détail du produit

Antigène: PFAS (Phosphoribosylformylglycinamidine Synthase (PFAS))

Type de proteíne: Recombinant

Attributs du protein: AA 1-1337

Origine: Souris

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette PFAS protéine est marqué à la Strep Tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA

Séquence: MAPVLHFYVR PSGHEGAASG RVFRRLQEKL PTLQSVETEL CYNVHWAAET LPWAEEMKKL MWLFGCPLVR DDVAQEPWLV PGSNDLLLEV GPRLNFSTPA STNIVSVCQA AGLRAVDRVE TTRRYRLSFT DHPTAEMEAI SLAALHDRMT EQHYPDPIQS FSPQSIPAPL KGSIDILAEG RPALEKANQE LGLALDSWDL DFYTKRFQEL QRNPSTVEVF DLAQSNSEHS RHWFFKGQLH VDGKKLAHSL FESIMSTQAS SNPNNVLKFC DNSSAIQGKK VKFLRPEDST RPSCFQQQQG LRHVVFTAET HNFPTGVAPF SGATTGTGGR IRDVQCTGRG AHVVAGTAGY CFGNLHIPDY NLPWEDPSFQ YPGNFARPLE VAIEASNGAS DYGNKFGEPV LAGFARSLGL QLPDGQRREW IKPIMFSGGI GSMEAKHVGK KPPEPGMEVV KVGGPVYRIG VGGGAASSVQ VQGDNTSDLD FGAVQRGDPE MEQKMNRVIR ACVEAPGGNP ICSLHDQGAG GNGNVLKELS DPEGAIIYTS RFQLGDPTLN ALEIWGAEYQ ESNALLLRPS DRDFLSRASA RERCPACFVG TITGDKRIVL VDDRECLVGK TGQGDAPLTP PTPVDLDLDW VLGKMPQKEF FLQRKPPVLQ PLALPPELSV RQALNRVLRL PAVASKRYLT NKVDRSVGGL VAQQQCVGPL QTPLADVAVV ALSHQECIGA ATALGEQPVK SLLDPKAAAR LAVSEALTNL VFALVTDLRD VKCSGNWMWA AKLPGEGAAL ADACEAMVAV MAALGVAVDG GKDSLSMAAR VGTETVQAPG SLVISAYAVC PDITATVTPD LKHPGGKGHL LYVPLSPGQH RLGGTALAQC FSQLGEHPPD LDLPENLVRA FHITQGLLKE CRLCSGHDVS DGGLVTCLLE MAFAGNCGIE VDVPAPGIHA LPVLFAEEPG LVLEVQEADV AGVRQRYESA GLRCLELGHT GEAGPQAMAR ISVNKAVVVE EPVGELRALW EETSFQLDLL QAEPRCVIEE KQGLKERTGP SYYLPPTFPV ASVPCKPGGP VPRVAILREE GSNGDREMAD AFHLAGFEVW DVTMQDLCSG AIRLDTFRGV AFVGGFSYAD VLGSAKGWAA AVTFNPQARE ELGRFRRRPD TFSLGVCNGC QLLALLGWVG SDPSEEQAEP GQDSQPTQPG LLLRHNLSGR FESRWATVRV EPGPALMLRG MEGSVLPVWS AHGEGYMAFS SPELQAKIEA KGLVPLHWAD DDGNPTEQYP LNPNGSPGGI AGICSQDGRH LALMPHPERA VRLWQWAWRP SPFDVLPTSP WLQLFINARN WTQEDSC
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: PFAS (Phosphoribosylformylglycinamidine Synthase (PFAS))

Autre désignation: Pfas (PFAS Produits)

Synonymes: FGAMS Protein, FGARAT Protein, PURL Protein, 4432409B16Rik Protein, Gm18 Protein, Sofa Protein, phosphoribosylformylglycinamidine synthase Protein, phosphoribosylformylglycinamidine synthase (FGAR amidotransferase) Protein, Spirs_3128 Protein, PFAS Protein, Pfas Protein

Sujet: Phosphoribosylformylglycinamidine synthase (FGAM synthase) (FGAMS) (EC 6.3.5.3) (Formylglycinamide ribonucleotide amidotransferase) (FGAR amidotransferase) (FGAR-AT) (Formylglycinamide ribotide amidotransferase),FUNCTION: Phosphoribosylformylglycinamidine synthase involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate (By similarity). {ECO:0000250}.

Poids moléculaire: 144.6 kDa

UniProt: Q5SUR0