TIAM1 Protein (AA 2-1591) (His tag)

N° du produit ABIN3135270

Détail du produit

Antigène: TIAM1 (T-Cell Lymphoma Invasion and Metastasis 1 (TIAM1))

Type de proteíne: Recombinant

Attributs du protein: AA 2-1591

Origine: Souris

Source: Cellules d'insectes

Purification/Conjugué: Cette TIAM1 protéine est marqué à la His tag.

Application: SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)

Séquence: GNAESQNVDH EFYGEKHASL GRKHTSRSLR LSHKTRRTRH ASSGKAIHRN SEVSTRSSST PSIPQSLAEN GLEPFSQEGA LDDFGDPIWV DRVDMGLRPV SYTDSSVTPS VDGSIVLTAA SVQSMPDSEE SRLYGDDATY LAEGGRRQCP YTSNGPTFME TASFKKKRSK SADIWREDSL EFSLSDLSQE HLTSNEEILG SAEEKDCEEA RGMETEASPR QLSTCQRANS LGDLYAQKNS GVKANGGPRN RFSSYCRNLV SDIPDLAKHK MPPAAAEETP PYSNYNTLPC RKSHCLSEGA TNPQISLSKS MQGRRAKTTQ DVNTGEGSEF ADSGIEGATT DTDLLSRRSN ATNSSYSPPT GRAFVGSDSG SSSTGDRARQ GVYENFRREL EMSTTNSESL EEAGSAHSDE QSSGTLSSPG QSDILLTAAQ GTVRKAGALA VKNFLVHKKN KKVESATRRK WKHYWVSLKG CTLFFYETDG RSGIDHNSVP KHAVWVENSI VQAVPEHPKK DFVFCLSNSL GDAFLFQTTS QTELENWITA IHSACAAAVA RHHHKEDTLR LLKSEIKKLE QKIDMDEKMK KMGEMQLSSV TDSKKKKTIL DQIFVWEQNL EQFQMDLFRF RCYLASLQGG ELPNPKRLLA FASRPTKVAM GRLGIFSVSS FHALVAARTG EIGVRRRTQA MSRSASKRRS RFSSLWGLDT TSKKKQGRPT INQVFGEGTD AVKRSLEGIF DDTVPDGKRE KEVVLPSVHQ HNPDCDIWVH EYFTPSWFCL PNNQPALTVV RPGDTARDTL ELICKTHQLD HSAHYLRLKF LMENRVQFYI PQPEEDIYEL LYKEIEICPK VTQNIHIEKS DAAADNYGFL LSSVDEDGIR RLYVNSVKET GLASKKGLKA GDEILEINNR AAGTLNSSML KDFLSQPSLG LLVRTYPEPE GGVELLENPP HRVDGPVDLG ESPLAFLTSN PGHSLSSEQG SSAETAPEEG EGPDLESSDE TDHSSKSTEQ VAAFCRSLHE MSPSDSSPSP QDATSPQLAT TRQLSDADKL RKVICELLET ERTYVKDLNC LMERYLKPLQ KETFLTQDEL DVLFGNLTEM VEFQVEFLKT LEDGVRLVPD LEKLEKVDQF KKVLFSLGGS FLYYADRFKL YSAFCASHTK VPKVLVKAKT DTAFKAFLDA QNPRQQHSST LESYLIKPIQ RVLKYPLLLR ELFALTDAES EEHYHLDVAI KTMNKVASHI NEMQKIHEEF GAVFDQLIAE QTGEKKEVAD LSMGDLLLHT SVIWLNPPAS LGKWKKEPEL AAFVFKTAVV LVYKDGSKQK KKLVGSHRLS IYEEWDPFRF RHMIPTEALQ VRALPSADAE ANAVCEIVHV KSESEGRPER VFHLCCSSPE SRKDFLKSVH SILRDKHRRQ LLKTESLPSA QQYVPFGGKR LCALKGARPA MSRAVSAPSK SLGRRRRRLA RNRFTIDSDA ISASSPEKEP QQPAGGGDTD RWVEEQFDLA QYEEQDDIKE TDILSDDDEF CESLKGASVD RDLQEQLQAA SISQRARGRR TLDSHASRMT QLKKQAALSG INGGLESASE EVIWVRREDF APSRKLNTEI
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Tiam1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Commentaires:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: TIAM1 (T-Cell Lymphoma Invasion and Metastasis 1 (TIAM1))

Autre désignation: Tiam1 (TIAM1 Produits)

Synonymes: TIAM1 Protein, tiam1 Protein, AI847750 Protein, D16Ium10 Protein, D16Ium10e Protein, T-cell lymphoma invasion and metastasis 1 Protein, T-cell lymphoma invasion and metastasis 1a Protein, T-cell lymphoma invasion and metastasis Protein, T cell lymphoma invasion and metastasis 1 Protein, TIAM1 Protein, tiam1a Protein, tiam1 Protein, Tiam1 Protein

Sujet: Modulates the activity of RHO-like proteins and connects extracellular signals to cytoskeletal activities. Acts as a GDP-dissociation stimulator protein that stimulates the GDP-GTP exchange activity of RHO-like GTPases and activates them. Activates RAC1, CDC42, and to a lesser extent RHOA. Required for normal cell adhesion and cell migration (By similarity). Affects invasiveness of T-lymphoma cells. {ECO:0000250}.

Poids moléculaire: 178.4 kDa Including tag.

UniProt: Q60610