HIRA Protein (AA 1-1015) (Strep Tag)

N° du produit ABIN3135444

Détail du produit

Antigène: HIRA (HIR Histone Cell Cycle Regulation Defective Homolog A (HIRA))

Type de proteíne: Recombinant

Attributs du protein: AA 1-1015

Origine: Souris

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette HIRA protéine est marqué à la Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Séquence: MKLLKPTWVN HNGKPIFSVD IHPDGTKFAT GGQGQDSGKV VIWNMSPVLQ EDDEKDENIP KMLCQMDNHL ACVNCVRWSN SGMYLASGGD DKLIMVWKRA TYIGPSTVFG SSGKLANVEQ WRCVSILRSH SGDVMDVAWS PHDAWLASCS VDNTVVIWNA VKFPEILATL RGHSGLVKGL TWDPVGKYIA SQADDRSLKV WRTLDWQLET SITKPFDECG GTTHVLRLSW SPDGHYLVSA HAMNNSGPTA QIIEREGWKT NMDFVGHRKA VTVVKFNPKI FKKKQKNGSS TKPSCPYCCC AVGSKDRSLS VWLTCLKRPL VVIHELFDKS IMDISWTLNG LGILVCSMDG SVAFLDFSQD ELGDPLSEEE KSRIHQSTYG KSLAIMTEAQ LSTAVIENPE MLKYQRRQQQ QQLDQKNATT RETSSASSVT GVVNGESLED IRKNLLKKQV ETRTADGRRR ITPLCIAQLD TGDFSTAFFN SIPLSSSLAG TMLSSPSGQQ LLPLDSSTPS FGASKPCTEP VAATSARPTG ESVSKDSMNA TSTPAASSPS VLTTPSKIEP MKAFDSRFTE RSKATPGAPS LTSVIPTAVE RLKEQNLVKE LRSRELESSS DSDEKVHLAK PSSLSKRKLE LEVETVEKKK KGRPRKDSRL LPMSLSVQSP AALSTEKEAM CLSAPALALK LPIPGPQRAF TLQVSSDPSM YIEVENEVTT VGGIRLSRLK CNREGKEWET VLSSRVLTAA GSCDVVCVAC EKRMLSVFST CGRRLLPPIL LPSPISTLHC TGPYVMALTA AATLSVWDVH RQVVVVKEES LHSILSGSDM TVSQILLTQH GIPVMNLSDG KAYCFNPSLS TWNLVSDKQD SLAQCADFRN SLPSQDAMLC SGPLAIIQGR TSNSGRQAAR LFSVPHVVQQ ETTLAYLENQ VAAALTLQSS HEYRHWLLLY ARYLVNEGFE YRLREICKDL LGPVHCSTGS QWESTVVGLR KRELLKELLP VIGQNLRFQR LFTECQEQLD ILRDK
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: HIRA (HIR Histone Cell Cycle Regulation Defective Homolog A (HIRA))

Autre désignation: Hira (HIRA Produits)

Synonymes: HIRA Protein, wu:fb97b07 Protein, wu:fi43b08 Protein, si:ch211-188g24.3 Protein, AA138857 Protein, D16Ertd95e Protein, N28177 Protein, Tuple1 Protein, DGCR1 Protein, TUP1 Protein, TUPLE1 Protein, dgcr1 Protein, hira-A Protein, tup1 Protein, tuple1 Protein, homolog of histone chaperone HIRA Protein, histone cell cycle regulator Protein, histone cell cycle regulator a Protein, protein HIRA Protein, HIR histone cell cycle regulation defective homolog A (S. cerevisiae) Protein, HIRA Protein, histone cell cycle regulator S homeolog Protein, histone chaperone HIRA-like protein Protein, Tuple1/HirA Protein, HIRA Protein, hira Protein, LOC100052845 Protein, LOC100352472 Protein, LOC100407742 Protein, LOC100450459 Protein, LOC100467668 Protein, LOC100581135 Protein, LOC486424 Protein, Hira Protein, LOC732736 Protein, hira.S Protein

Sujet: Protein HIRA (TUP1-like enhancer of split protein 1),FUNCTION: Required for the periodic repression of histone gene transcription during the cell cycle (By similarity). Cooperates with ASF1A to promote replication-independent chromatin assembly. Required for the formation of senescence-associated heterochromatin foci (SAHF) and efficient senescence-associated cell cycle exit. {ECO:0000250, ECO:0000269|PubMed:16399082}.

Poids moléculaire: 111.8 kDa

UniProt: Q61666

Pathways: Chromatin Binding