TOP2B Protein (AA 2-1612) (His tag)

N° du produit ABIN3135606

Détail du produit

Antigène: TOP2B (Topoisomerase (DNA) II beta 180kDa (TOP2B))

Type de proteíne: Recombinant

Attributs du protein: AA 2-1612

Origine: Souris

Source: Cellules d'insectes

Purification/Conjugué: Cette TOP2B protéine est marqué à la His tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Séquence: AKSSLAGSDG ALTWVNNATK KEELETANKN DSTKKLSVER VYQKKTQLEH ILLRPDTYIG SVEPLTQLMW VYDEDVGMNC REVTFVPGLY KIFDEILVNA ADNKQRDKNM TCIKVSIDPE SNIISIWNNG KGIPVVEHKV EKVYVPALIF GQLLTSSNYD DDEKKVTGGR NGYGAKLCNI FSTKFTVETA CKEYKHSFKQ TWMNNMMKTS EAKIKHFDGE DYTCITFQPD LSKFKMEKLD KDIVALMTRR AYDLAGSCKG VKVMFNGKKL PVNGFRSYVD LYVKDKLDET GVALKVIHEL ANERWDVCLT LSEKGFQQIS FVNSIATTKG GRHVDYVVDQ VVSKLIEVVK KKNKAGVSVK PFQVKNHIWV FINCLIENPT FDSQTKENMT LQPKSFGSKC QLSEKFFKAA SNCGIVESIL NWVKFKAQTQ LNKKCSSVKY SKIKGIPKLD DANDAGGKHS LECTLILTEG DSAKSLAVSG LGVIGRDRYG VFPLRGKILN VREASHKQIM ENAEINNIIK IVGLQYKKSY DDAESLKTLR YGKIMIMTDQ DQDGSHIKGL LINFIHHNWP SLLKHGFLEE FITPIVKASK NKQELSFYSI PEFDEWKKHI ENQKAWKIKY YKGLGTSTAK EAKEYFADME RHRILFRYAG PEDDAAITLA FSKKKIDDRK EWLTNFMEDR RQRRLHGLPE QFLYGTATKH LTYNDFINKE LILFSNSDNE RSIPSLVDGF KPGQRKVLFT CFKRNDKREV KVAQLAGSVA EMSAYHHGEQ ALMMTIVNLA QNFVGSNNIN LLQPIGQFGT RLHGGKDAAS PRYIFTMLSS LARLLFPAVD DNLLKFLYDD NQRVEPEWYI PIIPMVLING AEGIGTGWAC KLPNYDAREI VNNVRRMLEG LDPHPMLPNY KNFKGTIQEL GQNQYAVSGE IFVVDRNTVE ITELPVRTWT QVYKEQVLEP MLNGTDKTPA LISDYKEYHT DTTVKFVVKM TEEKLAQAEA AGLHKVFKLQ TTLTCNSMVL FDHMGCLKKY ETVQDILKEF FDLRLSYYGL RKEWLVGMLG AESTKLNNQA RFILEKIQGK ITIENRSKKD LIQMLVQRGY ESDPVKAWKE AQEKAAEEED SQNQHDDSSS DSGTPSGPDF NYILNMSLWS LTKEKVEELI KQRDTKGREV NDLKRKSPSD LWKEDLAAFV EELDKVEAQE REDILAGMSG KAIKGKVGKP KVKKLQLEET MPSPYGRRIV PEITAMKADA SRKLLKKKKG DPDTTVVKVE FDEEFSGTPA EGTGEETLTP SAPVNKGPKP KREKKEPGTR VRKTPTSTGK TNAKKVKKRN PWSDDESKSE SDLEEAEPVV IPRDSLLRRA AAERPKYTFD FSEEEDDDAA AADDSNDLEE LKVKASPITN DGEDEFVPSD GLDKDEYAFS SGKSKATPEK SSNDKKSQDF GNLFSFPSYS QKSEDDSAKF DSNEEDTASV FAPSFGLKQT DKLPSKTVAA KKGKPPSDTA PKAKRAPKQK KIVETINSDS DSEFGIPKKT TTPKGKGRGA KKRKASGSEN EGDYNPGRKP SKTASKKPKK TSFDQDSDVD IFPSDFTSEP PALPRTGRAR KEVKYFAESD EEEDVDFAMF N
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Top2b Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Commentaires:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: TOP2B (Topoisomerase (DNA) II beta 180kDa (TOP2B))

Autre désignation: Top2b (TOP2B Produits)

Synonymes: TOP2B Protein, D230016L12Rik Protein, Top-2 Protein, TOPIIB Protein, top2beta Protein, DNA topoisomerase II beta Protein, topoisomerase (DNA) II beta Protein, microRNA 4442 Protein, TOP2B Protein, Top2b Protein, MIR4442 Protein

Sujet: Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. {ECO:0000250}.

Poids moléculaire: 182.7 kDa Including tag.

UniProt: Q64511