CASKIN1 Protein (AA 1-1431) (Strep Tag)

N° du produit ABIN3135761

Détail du produit

Antigène: CASKIN1 (CASK Interacting Protein 1 (CASKIN1))

Type de proteíne: Recombinant

Attributs du protein: AA 1-1431

Origine: Souris

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette CASKIN1 protéine est marqué à la Strep Tag.

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Séquence: MGKEQELVQA VKAEDVGTAQ RLLQRPRPGK AKLLGSTKKI NVNFQDPDGF SALHHAALNG NTELISLLLE AQAAVDIKDN KGMRPLHYAA WQGRKEPMKL VLKAGSAVNV PSDEGHIPLH LAAQHGHYDV SEMLLQHQSN PCMVDNSGKT PLDLACEFGR VGVVQLLLSS NMCAALLEPR PGDTTDPNGT SPLHLAAKNG HIDIIRLLLQ AGIDINRQTK SGTALHEAAL CGKTEVVRLL LDSGINAQVR NTYSQTALDI VHQFTTSQAS KEIKQLLREA SAALQVRATK DYCNNYDLTS LNVKAGDIIT VLEQHPDGRW KGCIHDNRTG NDRVGYFPSS LGEAIVKRAG SRTGSEPSPP QGGGSLGPSA PPEEIWVLRK PFAGGDRSGS LSNVAGGRST GGHALHAGSE GVKLLATVLS QKSVSESSPG DSPVKPPEGS SGAARSQPPA AHAGQVYGEQ PPKKLESASA SASEGKSAEA VSQWLATFQL QLYAPNFTSA GYDLPTISRM TPEDLTAIGV TKPGHRKKIT AEISGLNIPD WLPEHKPANL AVWLSMIGLA QYYKVLVDNG YENIDFITDI TWEDLQEIGI TKLGHQKKLM LAVRKLAELQ KAEYSKYEGG PLRRKTPQSL EMMAIESPPP SEPAAAECQS PKMTTFQDSE LSGELQAALS GPAEAGAAAV EKSSNHLPPT PRTTSRESSL SGRARHISSS QELLGDGPPG PGSPMSRSQE YLLDEGMAPG TPPKEVRSSR HGHSVKRASV PPVPGKPRQV LPSGASHFTP PQTPTKAQPG SPQALGGPHG PATAKVKPTP QLLPPTDRPM SPRSLPQSPT HRGFAYVLPQ PVEGEVGPPA PGPAPPPVPA AVPTLCLPPE TDVEPGRPKK RAHSLNRYAA SDSEPERDEL LVPAAAGPYA TVQRRVGRSH SVRAPAGTDK NVNRSQSFAV RPRKKGPPPP PPKRSSSAMA SANLADEPAP DVEAEDGRLG VRAQRRRASD LAGSVDTGSA GSVKSIAAML ELSSIGGGGR AIRRPPEGHP TPRPASPEPG RVATVLASVK HKEAIGPDGE VVNRRRTLSG PVTGLLATAR RGSGEPAEQS HFMEDGTARQ RLRGPAKGEA SAEGPPLARV EASATLKRRI RAKQSQQENV KFILTESDTV KRRPKAKEPD TGPEPPPPLS VYQNGTATVR RRPTSEQAGP PELPPPPPPA EPPPADLMQL PPLPLPDGNA RKPVKPPVSP KPILSQPVSK IQGSPTPASK KVPLPGPGSP EVKRAHGTPP PVSPKPPPPP TAPKPAKALA GLQSSSATPS PVPSPARQPP AALIKPASSP PSQSASPVKP PSPGTPALHV PAKPPRAAAS VVSGPPVASD CASPGDSARQ KLEETSACLA AALQAVEEKI RQEDGQGPRP SSIEEKSTGS ILEDIGSMFD DLADQLDAML E
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: CASKIN1 (CASK Interacting Protein 1 (CASKIN1))

Autre désignation: Caskin1 (CASKIN1 Produits)

Synonymes: ANKS5A Protein, 3300002N10Rik Protein, C130061I24 Protein, C630036E02Rik Protein, mKIAA1306 Protein, CASK interacting protein 1 Protein, CASKIN1 Protein, Caskin1 Protein

Sujet: Caskin-1 (CASK-interacting protein 1),FUNCTION: May link the scaffolding protein CASK to downstream intracellular effectors. {ECO:0000250}.

Poids moléculaire: 150.5 kDa

UniProt: Q6P9K8