CEP120 Protein (AA 1-988) (Strep Tag)

N° du produit ABIN3135950

Détail du produit

Antigène: CEP120 (Centrosomal Protein 120kDa (CEP120))

Type de proteíne: Recombinant

Attributs du protein: AA 1-988

Origine: Souris

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette CEP120 protéine est marqué à la Strep Tag.

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Séquence: MVPKSDQLLI VVSILEGRHF PKRPKHLLVV EAKFDGEQLA TDPVDHTDQP EFATELAWEI DRKVLHQHRL QRTPIKLQCF ALDPQTSAKE TVGYIVLDLR TAQETKQAPK WYQLLSNKYT KFKAEVQISL TLETDTKAQV DSYKAKAAPP RDGKVLASLA GVDPKDIVAV LNEEGGYHQI GPAEHCTDPF ILSVTIAFAT QLEQLIPCTM KLPERQPEFF FYYSLLGNDV TNEPFSDLIN PNFEPERASV RIRSSVEILR VYLALHSKLQ IHLCCGDQSL GSTEIPLNGL LKKGSTEINQ HPVTVEGAFT LDPPNRAKQK LAPVPLDLAP TVGVSVALQR EGIDSQSLIE LKTQNGHEAE HSQKRVLTPI KEKTLTGPKS PRESPAPPPP PNQTPPTKDD ATESEVESLQ YDKDPKPTVK GIGSVPASLA QPEATCGASE VVTSGQKIAV PAASHHFCFS VDLRSVHDLE LSFPVNCILR YSYPFFGSAA PIMTNPPVEV RKNMEVFLPQ SYCAFDFATM PHQLQDTFLR IPLLVELWHK DKMSKDLLLG VARIQLSNIL SSEKTRFLGA NGEQCWRQTY SESVPVIAAQ GSNNRILDLS YTMTLEDYGL VKMREIFVSE SSQGVPAVDQ KPSSPPPAPC PSEIQMEPRE TLEYKAALEL EMWKEMQEDI FESQLKQKEL AHMQALAEEW KKRDRERESL VKKKVAEYSI LEGKLQKALT ELETREQQLA SAEAELQRER KELQLERERN LQELQDSVRR ARDDCVYQVE LERLKLKQLE EDKQRLQQQL NDAGNKYKTL EKEFQQFKDQ QNNKPEIRLQ SEINLLTLEK VELERKLESA TKSKLHYKQQ WGRALKELAR LKQREQESQM ARLKKQQEEL EQMRLRYLAA EEKETVRTEQ QELLDIRNEL NRLRQQEQNQ YQDCKEIASG KLGSPRGSGL EEGLDDYLTR LIEERDTLMR TGVYNHEDRI ISELDRQIRE VLTKNSAS
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: CEP120 (Centrosomal Protein 120kDa (CEP120))

Autre désignation: Cep120 (CEP120 Produits)

Synonymes: A230075C01 Protein, AU016693 Protein, Ccdc100 Protein, CCDC100 Protein, centrosomal protein 120 Protein, Cep120 Protein, CEP120 Protein

Sujet: Centrosomal protein of 120 kDa (Cep120) (Coiled-coil domain-containing protein 100),FUNCTION: Plays a role in the microtubule-dependent coupling of the nucleus and the centrosome. Involved in the processes that regulate centrosome-mediated interkinetic nuclear migration (INM) of neural progenitors and for proper positioning of neurons during brain development. Also implicated in the migration and selfrenewal of neural progenitors. Required for centriole duplication and maturation during mitosis and subsequent ciliogenesis. Required for the recruitment of CEP295 to the proximal end of new-born centrioles at the centriolar microtubule wall during early S phase in a PLK4-dependent manner (By similarity). {ECO:0000250|UniProtKB:Q8N960, ECO:0000269|PubMed:17920017, ECO:0000269|PubMed:20360068, ECO:0000269|PubMed:25251415}.

Poids moléculaire: 112.6 kDa

UniProt: Q7TSG1