CCDC40 Protein (AA 1-1192) (Strep Tag)

N° du produit ABIN3136175

Détail du produit

Antigène: CCDC40 (Coiled-Coil Domain Containing 40 (CCDC40))

Type de proteíne: Recombinant

Attributs du protein: AA 1-1192

Origine: Souris

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette CCDC40 protéine est marqué à la Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Séquence: MMDAEKVSTD GEAISEGEVG SNGETPPETE VEFIGETAPD TDVEFIGETS PGTDVEPTGE SIQETEVESI GEATPGMDVE PIKKTMTELN VESIGEETSE TDVDSIRKAL RGIDLESITV AYPPKKAKHR KVRPQAEVES TGRAAPEGEL EVSDHEKVEA LLDELDELSE IVSSPEVSYS DISPLEMGED DTNVSATSTD TFQQGIYEPI EPIEPTEPPE PAEPPKPAET PEDSTVRAPA HPYQRDFPMG ARHRFRLSIM GSLTPSDTDD LPLETDEPPQ QESVQSTPRA LEETRIQFLD QVQSLSPEAL LDRATEGSDE AEEEGSQLIV LDPDHPLMIR FQEALKGYLN RQMDKLKLDV QELDVATKQT RSQRQELGVN LYGVQQHLAR LQMQLEKSHD RHSLVACERR RKEEELQCAR SVYNKTCQTA NEERKKLAAL QTEVESLALH LFYMQNIEQD VRDDIQVMKQ VVRKTETEKM HAEVEKKKQD LFVDQLTERS HQLEENIALF EAQYLSQAED TRVLKKAVTE AITEIDTIAV EKKRILQQWT TSLVGMKHRN EAYKTVMDAL RECQHQVKST DSEIEVCKKS IMQEEEKNEK LARLLNRAET EATLVQKMTA QCLSKQEALQ TEFNTYQLAL QDTEEMLNKG YVEHSAVLSE LQATRQAFHQ EQELRQKMDM SMVDKLQEQG TSSKMTKYFH QLLRKLQKEN TNLVTHLSKI DGDIAQATLD ITNTNCKIDM HKKTLAEMDK EVKRFNDLIT NSESEIARRT ILIERKQSLI NFFNKQLEQM VSELGGEEAG PLELEIKRLS KLTEEYNTGV AEAQMTWLRL QQELVQVTHE REEQLVSVDQ LKKEVHIMEQ KKLRIESKIA HEKKEQKIVS RHMRDLDNDL SKLNMLLDKN RCSSEELEQN NIATETEFLR TLKDSERETI QMQEKLMELS EEKATLLNSF MEAEHQIMLW EKKIQLAKEM RSSVDSETGQ TEIRAMKAEI HRMKVRHGQL LKQQEKMIRD MELAVARRET IVVQAEGQSK IDKKVITKTE FHYQQRELQK KVREMHKATD DCTNTISELE ETQKFLSSSL QEKQQLLSEM QATTDVLEEE INQLTALKRQ NLLEIVTLQT RGKHLQAAIE GKYVFLHRNS RSQLMERKRL SVRLSQLNKV LSSVQEDYPQ YQEVLQSIQQ KIATKLETPE PS
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: CCDC40 (Coiled-Coil Domain Containing 40 (CCDC40))

Autre désignation: Ccdc40 (CCDC40 Produits)

Synonymes: CILD15 Protein, B930008I02Rik Protein, im:7149866 Protein, lok Protein, si:dkey-237i9.5 Protein, wu:fb11d02 Protein, coiled-coil domain containing 40 Protein, CCDC40 Protein, Ccdc40 Protein, ccdc40 Protein

Sujet: Coiled-coil domain-containing protein 40 (Protein links),FUNCTION: Required for assembly of dynein regulatory complex (DRC) and inner dynein arm (IDA) complexes, which are responsible for ciliary beat regulation, thereby playing a central role in motility in cilia and flagella. Probably acts together with CCDC39 to form a molecular ruler that determines the 96 nanometer (nm) repeat length and arrangements of components in cilia and flagella. Not required for outer dynein arm complexes assembly. Required for axonemal recruitment of CCDC39. {ECO:0000250|UniProtKB:A8IQT2, ECO:0000250|UniProtKB:Q4G0X9}.

Poids moléculaire: 136.8 kDa

UniProt: Q8BI79