TBC1D4 Protein (AA 1-1307) (Strep Tag)

N° du produit ABIN3136316

Détail du produit

Antigène: TBC1D4 (TBC1 Domain Family, Member 4 (TBC1D4))

Type de proteíne: Recombinant

Attributs du protein: AA 1-1307

Origine: Souris

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette TBC1D4 protéine est marqué à la Strep Tag.

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Séquence: MESPSCIQDE PFPHPLEPEP SAPAQPGATK PGDKRFRLWY VGGSCLDRRT TLPMLPWLMA EIRRRSQKPD AGGCGAPAAR EVILVLSAPF LRCVPAPGAG VGGGAGSGAV QPNTGVFIFE HKAQHISRFI HNSHDLTYFA YLIKAQPDDP ESQMACHVFR ATDPNQVPDV ISSIRQLSKA AMKEDSKPSK DNEDAFYNSQ KFEVLYCGRV IVTHKKAPSS LIDDCKDKFS LHEQQRLKLQ GERGGDPGDE MGVLEVESPV SPDDSLPEKA DGTVNSPRAL PSLASLPALA SQPALASSRV CFPERILEDC GFDEQQEFRS RCSSVTGVMQ KKVHENNQKT QPRRRHASAP SHVQPSDSEK NRTMLFQVGR FEINLISPDT KSVVLEKNFK DISSCSQGIK HVDHFGFICR ESPEPGLSQY ICYVFQCANE SLVDEVMLTL KQAFSTAAAL QSAKTQIKLC ETCPMHSLHK LCERIEGLYP PRAKLVIQRH LSSLTDNEQA DIFERVQKMK PISDQEENEL VILHLRQLCE AKQRTHVHIG EGPAIISNST IPENVTSGGR FKLDVLKNKA KRSLTSSLEN IFSRGANRMR GRLGSMDSFE RANSLASEKD FSPGDSPPGT PPASPLSSAW HAFPEEDSDS PQFRRRAHTF SHPPSSSRRK LNLQDGKAHG LRSPLLRQSS SEQCSIVPSA RRMYKESNSS CSLPSLHTSF SAPSFTAPSF LKSFYQNSGR LSPQYENEIR QDTASESSDG EGRKRTSSTC SNESLNAGGT PVTPRRVSWR QRIFLRVASP VNKSPSAMQQ QKDGLDRTEL LPLSPLSPTM EEEPLIIFLS GDEDTEKVEE KKKSKELKSL WKKAIHQQIL LLRMEKENQK LEEARRDELQ SRKVKLDYEE VGTCQKEILI AWDKKLLNCR TKIRCDMEDI HTSLKEGVPK SRRGEIWQFL ALQYRLRHRL PNKHQPPDTS YKELLKQLTA QQHAILVDLG RTFPTHPYFS VQLGAGQLSL FNLLKAYSLL DKEVGYCQGI SFVAGVLLLH MSEEQAFEML KFLMYDLGFR KQYRPDMMSL QIQMYQLSRL LHDYHRELYN HLEENEISPS LYAAPWFLTL FASQFPLGFV ARVFDIIFLQ GTEVIFKVAL SLLSSQEALI MECENFENIV EFLKSTLPDM NTTEMEKIIT QVFEMDISKQ LHAYEVEYHV LQDELLESSY ACEDNESLEK LERANNQLKR QNMDLLEKLQ VAHAKIQALE SNLETLLTRE TKMKALIRTL EQDKMAYQKT VEQIRKLLPA DALANCELLL KDLTHPTNDK AKAGNKP
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: TBC1D4 (TBC1 Domain Family, Member 4 (TBC1D4))

Autre désignation: Tbc1d4 (TBC1D4 Produits)

Synonymes: AS160 Protein, 5930406J04Rik Protein, A930035N22 Protein, AV295684 Protein, As160 Protein, RGD1561609 Protein, TBC1 domain family member 4 Protein, TBC1 domain family, member 4 Protein, TBC1D4 Protein, Tbc1d4 Protein

Sujet: TBC1 domain family member 4 (Akt substrate of 160 kDa) (AS160),FUNCTION: May act as a GTPase-activating protein for RAB2A, RAB8A, RAB10 and RAB14. Promotes insulin-induced glucose transporter SLC2A4/GLUT4 translocation at the plasma membrane, thus increasing glucose uptake (By similarity). {ECO:0000250}.

Poids moléculaire: 147.5 kDa

UniProt: Q8BYJ6