MTMR3 Protein (AA 1-1196) (Strep Tag)

N° du produit ABIN3136550

Détail du produit

Antigène: MTMR3 (Myotubularin Related Protein 3 (MTMR3))

Type de proteíne: Recombinant

Attributs du protein: AA 1-1196

Origine: Souris

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette MTMR3 protéine est marqué à la Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Séquence: MDEEMRHSLE CIQANQIFPR KQLIREDENL QVPFLELHGE STEYVGRAEE AIIALSNYRL HIKFKESLVN VPLQLIESVE CRDIFQLHLT CKDCKVIRCQ FPTFEQCQDW LKRLNNAIRP PGKIEDLFSF AYHAWCMEVY ASEKEQHGDL CRPGEHVTSR FKNEVERMGF DMNNAWRISN INEKYKLCGS YPQELIVPAW ITDKELESVA GFRSWKRIPA VIYRHQSNGA VIARCGQPEV SWWGWRNADD EHLVQSVARA CASDSQSSIS KVSTRNSCRD FPNAGDLSDV EFDSSLSNTS GAESLALQPQ KLLILDARSY AAAVANRAKG GGCECPEYYP NCEVVFMGMA NIHSIRRSFQ SLRLLCTQMP DPGNWLSALE STKWLHHLSV LLKSALLVVH AVDRDQRPVL VHCSDGWDRT PQIVALAKLL LDPYYRTIEG FQVLVEMEWL DFGHKFADRC GHGEDSDDLN ERCPVFLQWL DCVHQLQRQF PCSFEFNEAF LVKLVQHTYS CLFGTFLCNN AKERGEKQTQ ERTCSVWSLL RAGNKAFKNL LYSSQSEAVL YPVCHVRNLM LWSAVYLPCP SPSTPTDDSC APYPVPGTSP DEPPLSRLPK TRSFDNLTTT CENMVPLASR RSSDPSLNEK WQEHGRSLEL SSFASAGEEV PAMDSLRKPS RLLGGAELSV AAGVAEGQME NILQEATKEE SGVEEPTHRG HTEVPEVKEE APLAKESSMA AEGPVVLYQE PQLDDATLRS HQGPSLSLFS QGIPEHQDGH NVLSSSLQAP LRGEDSQEVP VEQPQVENIA EDRENVAPAV PVDAKVGLGI SQSSSLLPSQ VPFETRGPHI NNSVHMLLED KVKSESGPQL HHRPCPASSG RFSGKDMLPV APEPRSAERP QWDSVLHRTS SPGNTLSLLQ APCALPLDKC RQGIVCNGAL ETENKASEQP AGFDTLQKYP TPNGHCANWE AGRSKDSLSH QLSATSCSSA HLYSRNLHHK WLNSHSGRPS TTSSPDQPSR SHLDDDGMPV YTDTIQQRLR QIESGHQQEV ETLKKQVQEL KSRLESQYLT SSLRFNGDFG DEVTSIPDSE SNLDQNCVSR CSTEIFSEAS WEQVDKQDTE MTRWLPDHLA AHCYACDSAF WLASRKHHCR NCGNVFCSSC CNQKVPVPSQ QLFEPSRVCK SCYSSLHPTS SSIDLELDKP IAATSN
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: MTMR3 (Myotubularin Related Protein 3 (MTMR3))

Autre désignation: Mtmr3 (MTMR3 Produits)

Synonymes: MGC68981 Protein, MGC143436 Protein, DKFZp459K2113 Protein, FYVE-DSP1 Protein, ZFYVE10 Protein, 1700092A20Rik Protein, AI255150 Protein, AW557713 Protein, mKIAA0371 Protein, myotubularin-related protein 3 Protein, myotubularin related protein 3 Protein, myotubularin related protein 3 L homeolog Protein, mtmr3 Protein, MTMR3 Protein, mtmr3.L Protein, Mtmr3 Protein

Sujet: Myotubularin-related protein 3 (EC 3.1.3.48) (Phosphatidylinositol-3,5-bisphosphate 3-phosphatase) (EC 3.1.3.95) (Phosphatidylinositol-3-phosphate phosphatase) (EC 3.1.3.64),FUNCTION: Phosphatase that acts on lipids with a phosphoinositol headgroup. Has phosphatase activity towards phosphatidylinositol 3-phosphate and phosphatidylinositol 3,5-bisphosphate. May also dephosphorylate proteins phosphorylated on Ser, Thr, and Tyr residues (By similarity). {ECO:0000250|UniProtKB:Q13615}.

Poids moléculaire: 133.8 kDa

UniProt: Q8K296

Pathways: Inositol Metabolic Process