ERRFI1 Protein (AA 2-461) (His tag)

N° du produit ABIN3136934

Détail du produit

Antigène: ERRFI1 (ERBB Receptor Feedback Inhibitor 1 (ERRFI1))

Type de proteíne: Recombinant

Attributs du protein: AA 2-461

Origine: Souris

Source: Cellules d'insectes

Purification/Conjugué: Cette ERRFI1 protéine est marqué à la His tag.

Application: ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)

Séquence: STAGVAAQDI RVPLKTGFLH NGQALGNMKS CWGSHSEFEN NFLNIDPITM AYNLNSPAQE HLTTVGCAAR SAPGSGHFFA ECGPSPRSSL PPLVISPSES SGQREEDQVM CGFKKLSVNG VCTSTPPLTP IKSCPSPFPC AALCDRGSRP LPPLPISEDL CVDEADSEVE LLTTSSDTDL LLEDSAPSDF KYDAPGRRSF RGCGQINYAY FDSPTVSVAD LSCASDQNRV VPDPNPPPPQ SHRRLRRSHS GPAGSFNKPA IRISSCTHRA SPSSDEDKPE VPPRVPIPPR PAKPDYRRWS AEVTSNTYSD EDRPPKVPPR EPLSRSNSRT PSPKSLPSYL NGVMPPTQSF APDPKYVSSK ALQRQSSEGS ANKVPCILPI IENGKKVSST HYYLLPERPP YLDKYEKYFK EAEETNPSTQ IQPLPAACGM ASATEKLASR MKIDMGSHGK RKHLSYVVSP
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Errfi1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Commentaires:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: ERRFI1 (ERBB Receptor Feedback Inhibitor 1 (ERRFI1))

Autre désignation: Errfi1 (ERRFI1 Produits)

Synonymes: GENE-33 Protein, MIG-6 Protein, MIG6 Protein, RALT Protein, 1300002F13Rik Protein, AI788755 Protein, Mig-6 Protein, Mig6 Protein, RGD1307599 Protein, Ralt Protein, Xxx Protein, gene 33 Protein, ERRFI1 Protein, fi40f02 Protein, MGC162129 Protein, wu:fi40f02 Protein, ERBB receptor feedback inhibitor 1 Protein, ERBB receptor feedback inhibitor 1 S homeolog Protein, ERBB receptor feedback inhibitor 1a Protein, ERRFI1 Protein, Errfi1 Protein, errfi1.S Protein, errfi1a Protein, errfi1 Protein, errfi Protein

Sujet: Negative regulator of EGFR signaling in skin morphogenesis. Acts as a negative regulator for several EGFR family members, including ERBB2, ERBB3 and ERBB4. Inhibits EGFR catalytic activity by interfering with its dimerization. Inhibits autophosphorylation of EGFR, ERBB2 and ERBB4. Important for normal keratinocyte proliferation and differentiation. Plays a role in modulating the response to steroid hormones in the uterus. Required for normal response to progesterone in the uterus and for fertility. Mediates epithelial estrogen responses in the uterus by regulating ESR1 levels and activation. Important for regulation of endometrium cell proliferation. Important for normal prenatal and perinatal lung development. {ECO:0000269|PubMed:16648858, ECO:0000269|PubMed:19439667, ECO:0000269|PubMed:19683494, ECO:0000269|PubMed:19710174, ECO:0000269|PubMed:20018910}.

Poids moléculaire: 50.9 kDa Including tag.

UniProt: Q99JZ7

Pathways: EGFR Signaling Pathway