IGF2BP3 Protein (AA 1-579) (Strep Tag)

N° du produit ABIN3137015

Détail du produit

Antigène: IGF2BP3 (Insulin-Like Growth Factor 2 mRNA Binding Protein 3 (IGF2BP3))

Type de proteíne: Recombinant

Attributs du protein: AA 1-579

Origine: Souris

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette IGF2BP3 protéine est marqué à la Strep Tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA

Séquence: MNKLYIGNLS DHAGPADLES VFKDAKIPVA GPFLVKTGYA FVDCPDEGWA LKAIEALSGK MELHGKPMEV EHSVPKRQRI RKLQIRNIPP HLQWEVLDSL LVQYGVVESC EQVNTDSETA VVNVTYSSKD QARQALDKLN GFQLENFTLK VAYIPDETAA QQNPSPQLRG RRGPGQRGSS RQASPGSVSK QKPCDLPLRL LVPTQFVGAI IGKEGATIRN ITKQTQSKID VHRKENTGAA EKSITILSTP EGTSAACKSI LEIMHKEAQD IKFTEEIPLK ILAHNNFVGR LIGKEGRNLK KIEQDTDTKI TISPLQELTL YNPERTITVK GSVETCAKAE EEIMKKIRES YENDIASMNL QAHLIPGLNL NALGLFPPTS GMPPPTSGPP STLTPPYPQF EQSETETVHL FIPALSVGAI IGKQGQHIKQ LSRFAGASIK IAPAEAPDAK VRMVIITGPP EAQFKAQGRI YGKIKEENFV SPKEEVKLEA HIRVPSFAAG RVIGKGGKTV NELQSLSSAE VVVPRDQTPD ENDQVVVKIT GHFYACQVAQ RKIQEILTQV KQHQQQKALQ SGPPQSRRK
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: IGF2BP3 (Insulin-Like Growth Factor 2 mRNA Binding Protein 3 (IGF2BP3))

Autre désignation: Igf2bp3 (IGF2BP3 Produits)

Synonymes: CT98 Protein, IMP-3 Protein, IMP3 Protein, KOC1 Protein, VICKZ3 Protein, Ab2-255 Protein, RGD1306512 Protein, imp3 Protein, koc1 Protein, vera Protein, imp-3 Protein, vg1rbp Protein, vickz3 Protein, 2610101N11Rik Protein, AA522010 Protein, AL022933 Protein, AU045931 Protein, Koc13 Protein, Neilsen Protein, mimp3 Protein, dvr1rbp Protein, wu:fb79c12 Protein, wu:fe06h09 Protein, zVg1RBP Protein, B3 Protein, IGF2 mRNA-binding protein 3-B Protein, IMP Protein, IMP-3-B Protein, Vg1 RBP-B Protein, Vg1-RBP Protein, igf2bp3 Protein, igf2bp3-a Protein, igf2bp3-b Protein, IGF2 mRNA-binding protein 3-A Protein, IMP-3-A Protein, Vg1 RBP-A Protein, vera-A Protein, insulin like growth factor 2 mRNA binding protein 3 Protein, insulin-like growth factor 2 mRNA binding protein 3 Protein, insulin like growth factor 2 mRNA binding protein 3 S homeolog Protein, insulin like growth factor 2 mRNA binding protein 3 L homeolog Protein, IGF2BP3 Protein, Igf2bp3 Protein, igf2bp3 Protein, igf2bp3.S Protein, igf2bp3.L Protein

Sujet: Insulin-like growth factor 2 mRNA-binding protein 3 (IGF2 mRNA-binding protein 3) (IMP-3) (mIMP-3) (IGF-II mRNA-binding protein 3) (VICKZ family member 3),FUNCTION: RNA-binding factor that may recruit target transcripts to cytoplasmic protein-RNA complexes (mRNPs). This transcript 'caging' into mRNPs allows mRNA transport and transient storage. It also modulates the rate and location at which target transcripts encounter the translational apparatus and shields them from endonuclease attacks or microRNA-mediated degradation. Preferentially binds to N6-methyladenosine (m6A)-containing mRNAs and increases their stability (By similarity). Binds to the 3'-UTR of CD44 mRNA and stabilizes it, hence promotes cell adhesion and invadopodia formation (By similarity). Binds to beta-actin/ACTB and MYC transcripts (By similarity). Increases MYC mRNA stability by binding to the coding region instability determinant (CRD) and binding is enhanced by m6A-modification of the CRD (By similarity). Binds to the 5'-UTR of the insulin-like growth factor 2 (IGF2) mRNAs. {ECO:0000250|UniProtKB:O00425, ECO:0000269|PubMed:15753088}.

Poids moléculaire: 63.6 kDa

UniProt: Q9CPN8