ARHGEF25/GEFT Protein (AA 1-618) (Strep Tag)

N° du produit ABIN3137047

Détail du produit

Antigène: ARHGEF25/GEFT (ARHGEF25) (Rho Guanine Nucleotide Exchange Factor 25 (ARHGEF25))

Type de proteíne: Recombinant

Attributs du protein: AA 1-618

Origine: Souris

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette ARHGEF25/GEFT protéine est marqué à la Strep Tag.

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Séquence: MKPPDRPTPG RTDRILGVMG GMLRACAVPG QEGPPERDPL GPGSTKTESD CIEEDQTGQR EPEVLAWAPQ PESYSIAVSE GSMSASAVSG LAALSGPSSG LSSDPCSPIP PGPVTGLRRW LDHSKHCLSV ETEAESGQTG QCENWTLGPT LTTGQELPEL TLLTTLLEGP GDKAQPAEEE TLSQAPKNEE EQKKMALERS MFVLGELVET ERTYEDDLGQ IVEGYMATMA TQGVPESLRG RDRIVFGNIQ QIYEWHRDYF LQELQQCLKD PDWLAQLFIK HERRLHMYVV YCQNKPKSEH VLSEFGDSYF EELRQQLGHR LQLNDLLIKP VQRIMKYQLL LKDFLKYYRR AGKDTEELEQ AVEVMCFVPK RCNDMMSLGR LRGFEGKLTA QGKLLGQDTF LVTEPEAGGL LSSRGRERRV FLFEQIIIFS EALGGGGRGG AQPGYVYKNS IKVSCLGLEG NLQGNPCRFA LTSRGPEGGI QRYVLQASDP AVSQAWIKQV AQILESQRDF LNALQSPIEY QRRESQTNSL GRPGGPWVGS PGRMRPGDLA QASMHTPING SLPSLLLLPR GEVSRVLLPL DTQALSDTPQ TPHDSPALPT VNTPPCQARL AKLDEDEL
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: ARHGEF25/GEFT (ARHGEF25) (Rho Guanine Nucleotide Exchange Factor 25 (ARHGEF25))

Autre désignation: Arhgef25 (ARHGEF25 Produits)

Synonymes: GEFT Protein, p63RhoGEF Protein, 2410008H17Rik Protein, D10Ertd610e Protein, Geft Protein, Rho guanine nucleotide exchange factor 25 Protein, Rho guanine nucleotide exchange factor (GEF) 25 Protein, ARHGEF25 Protein, Arhgef25 Protein

Sujet: Rho guanine nucleotide exchange factor 25 (Guanine nucleotide exchange factor GEFT) (Rac/Cdc42/Rho exchange factor GEFT) (RhoA/Rac/Cdc42 guanine nucleotide exchange factor GEFT) (p63RhoGEF),FUNCTION: May play a role in actin cytoskeleton reorganization in different tissues since its activation induces formation of actin stress fibers. It works as a guanine nucleotide exchange factor for Rho family of small GTPases. Links specifically G alpha q/11-coupled receptors to RHOA activation (By similarity). May be an important regulator of processes involved in axon and dendrite formation. In neurons seems to be an exchange factor primarily for RAC1. Involved in skeletal myogenesis. {ECO:0000250, ECO:0000269|PubMed:15322108, ECO:0000269|PubMed:16314529, ECO:0000269|PubMed:16496360}.

Poids moléculaire: 68.3 kDa

UniProt: Q9CWR0