EIF4ENIF1 Protein (AA 1-983) (Strep Tag)

N° du produit ABIN3137234

Détail du produit

Antigène: EIF4ENIF1 (Eukaryotic Translation Initiation Factor 4E Nuclear Import Factor 1 (EIF4ENIF1))

Type de proteíne: Recombinant

Attributs du protein: AA 1-983

Origine: Souris

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette EIF4ENIF1 protéine est marqué à la Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Séquence: MEKSVAETEN GDAFLELKKL PTSKSPHRYT KEELLDIKER PYSKQRPSCL SEKYDSDGVW DPEKWHASLY PASGRSSPVE SLKKESESDR PSLVRRIADP RERVKEDDLD VVLSPQRRSF GGGCHVTAAV SSRRSGSPLE KDSDGLRLLG GRRIGSGRII SARAFEKDHR LSDKDLRDLR DRDRERDYKD KRFRREFGDS KRVFGERRRN DSYTEEEPEW FSAGPTSQSE TIELTGFDDK ILEEDHKGRK RTRRRTASVK EGIVECNGGV AEEDEVEVIL AQEPSADQEV PRDVILPEQS PGEFDFNEFF NLDKVPCLAS MIEDVLGEGS VSASRFSRWF SNPSRSGSRS SSLGSTPHEE LERLAGLEQA VLSPGQNSGN YFAPIPSEDH AENKVDILEM LQKAKVDLKP LLSSLSANKE KLKESSHSGV VLSVEEVEAG LKGLKVDQQM KNSTPFMAEH LEETLSAASS NRQLKKDGDM TAFNKLVNTM KASGTLPTQP KVSRNVESHL LAPAEIPGQP VSKNILQELL GQPVQRPASS NLLSGLMGSL EATASLLSQR APSPPMSQVF RTQAASADYL HPRIPSPIGF PSGPQQLLGD PFQGMRKPMS PVSAQMSQLE LQQAALEGLA LPHDLAVQTA PFYQPGFSKP QVDRTRDGLR NRQQRMSKSP APMHGGNSSS PAPAASITSM LSPSFTPTSV IRKMYESREK TKEEMAPGMV VPGDGKEDTQ KTSEENLLSS NPIPNTDQDS STTNPKLSTL QRSSCSTPLS QTSRYTKEQD YRPKTAGRKT PTLASPVPGT PFLRPTHQVP LVPHVPIVRP AHQLHPGLVQ RLIAQGVHPQ HLPSLLQAGV LPPGIDMAPL QGLSGPLLGQ PLYPLVSAAS HPLLNPRPGT PLHLAVMQQQ LQRSVLHPPG SSSQAAAISV QTPQNVPSRS GMPHMHSQLE HRTSQRSSSP VGLAKWFGSD VLQQPLPSMP TKVISVDELE YRQ
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: EIF4ENIF1 (Eukaryotic Translation Initiation Factor 4E Nuclear Import Factor 1 (EIF4ENIF1))

Autre désignation: Eif4enif1 (EIF4ENIF1 Produits)

Synonymes: RGD1560908 Protein, eif4enif1 Protein, MGC80355 Protein, EIF4ENIF1 Protein, wu:fd15a05 Protein, zgc:101646 Protein, MGC147579 Protein, 4E-T Protein, Clast4 Protein, 2610509L04Rik Protein, A930019J01Rik Protein, AA410001 Protein, AU021239 Protein, D11Ertd166e Protein, eukaryotic translation initiation factor 4E nuclear import factor 1 Protein, eukaryotic translation initiation factor 4E nuclear import factor 1 S homeolog Protein, Eif4enif1 Protein, EIF4ENIF1 Protein, eif4enif1.S Protein, eif4enif1 Protein

Sujet: Eukaryotic translation initiation factor 4E transporter (4E-T) (eIF4E transporter) (CD40 ligand-activated specific transcript 4) (Eukaryotic translation initiation factor 4E nuclear import factor 1),FUNCTION: EIF4E-binding protein that regulates translation and stability of mRNAs in processing bodies (P-bodies) (PubMed:25456498). Plays a key role in P-bodies to coordinate the storage of translationally inactive mRNAs in the cytoplasm and prevent their degradation (By similarity). Acts as a binding platform for multiple RNA-binding proteins: promotes deadenylation of mRNAs via its interaction with the CCR4-NOT complex, and blocks decapping via interaction with eIF4E (EIF4E and EIF4E2), thereby protecting deadenylated and repressed mRNAs from degradation (By similarity). Component of a multiprotein complex that sequesters and represses translation of proneurogenic factors during neurogenesis (PubMed:25456498). Promotes miRNA-mediated translational repression (By similarity). Involved in mRNA translational repression mediated by the miRNA effector TNRC6B by protecting TNRC6B-targeted mRNAs from decapping and subsequent decay (By similarity). Required for the formation of P-bodies (By similarity). Also acts as a nucleoplasmic shuttling protein, which mediates the nuclear import of EIF4E and DDX6 by a piggy-back mechanism (By similarity). {ECO:0000250|UniProtKB:Q9NRA8, ECO:0000269|PubMed:25456498}.

Poids moléculaire: 108.0 kDa

UniProt: Q9EST3