SLC26A2 Protein (AA 1-739) (Strep Tag)

N° du produit ABIN3123161

Détail du produit

Antigène: SLC26A2 (Solute Carrier Family 26 (Sulfate Transporter), Member 2 (SLC26A2))

Type de proteíne: Recombinant

Attributs du protein: AA 1-739

Origine: Souris

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette SLC26A2 protéine est marqué à la Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Séquence: MSSENKEQHD LSPRDLPEEA FGFPSELPLE TQRRSGTDLR QSETGHGRRA FRRIHMELRE KPDTDIKQFV IRELQKSCQC SAAKVRDGAF DFFPVLRWLP KYDLKKNILG DVMSGLIVGI LLVPQSIAYS LLAGQEPIYG LYTSFFASII YFLFGTSRHI SVGIFGILCL MIGEVVDREL HKACPDTDAT SSSIAVFSSG CVVVNHTLDG LCDKSCYAIK IGSTVTFMAG VYQVAMGFFQ VGFVSVYLSD ALLSGFVTGA SFTILTSQAK YLLGLSLPRS HGVGSVITTW IHIFRNIRNT NICDLITSLL CLLVLVPSKE LNEHFKDKLK APIPVELIVV VAATLASHFG KLNGNYNSSI AGHIPTGFMP PKAPDWSLIP NVAVDAIAIS IIGFAITVSL SEMFAKKHGY TVKANQEMYA IGFCNIIPSF FHCITTSAAL AKTLVKESTG CQTQLSAIVT ALVLLLVLLV IAPLFYSLQK CVLGVITIVN LRGALLKFRD LPKMWRLSRM DTVIWFVTML SSALLSTEIG LLVGVCFSMF CVILRTQKPK NSLLGLEEES ETFESISTYK NLRSKSGIKV FRFIAPLYYI NKECFKSALY KKALNPVLVK AAWKKAAKRK LKEEMVTFRG DPDEVSMQLS HDPLEVHTIV IDCSAIQFLD TAGIHTLKEV RRDYEAVGIQ VLLAQCNPSV RDSLARGEYC KKEEETLLFY SLSEAVAFAE DSQNQKGVCV VNGLSLSGD
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: SLC26A2 (Solute Carrier Family 26 (Sulfate Transporter), Member 2 (SLC26A2))

Autre désignation: Slc26a2 (SLC26A2 Produits)

Synonymes: SLC26A2 Protein, D5S1708 Protein, DTD Protein, DTDST Protein, EDM4 Protein, MST153 Protein, MSTP157 Protein, Dtdst Protein, Dtd Protein, ST-OB Protein, solute carrier family 26 member 2 Protein, sulfate transporter Protein, solute carrier family 26 (sulfate transporter), member 2 Protein, SLC26A2 Protein, LOC100544098 Protein, LOC100556372 Protein, Slc26a2 Protein

Sujet: Sulfate transporter (Diastrophic dysplasia protein homolog) (ST-OB) (Solute carrier family 26 member 2),FUNCTION: Sulfate transporter which mediates sulfate uptake into chondrocytes in order to maintain adequate sulfation of proteoglycans which is needed for cartilage development (PubMed:24302720). Mediates electroneutral anion exchange of sulfate ions for oxalate ions, sulfate and oxalate ions for chloride and/or hydroxyl ions and chloride ions for bromide, iodide and nitrate ions (PubMed:20219950, PubMed:22190686, PubMed:24302720). The coupling of sulfate transport to both hydroxyl and chloride ions likely serves to ensure transport at both acidic pH when most sulfate uptake is mediated by sulfate-hydroxide exchange and alkaline pH when most sulfate uptake is mediated by sulfate-chloride exchange (PubMed:22190686). Essential for chondrocyte proliferation, differentiation and cell size expansion (PubMed:24302720). {ECO:0000269|PubMed:20219950, ECO:0000269|PubMed:22190686, ECO:0000269|PubMed:24302720}.

Poids moléculaire: 81.6 kDa

UniProt: Q62273

Pathways: Glycosaminoglycan Metabolic Process, Ribonucleoside Biosynthetic Process