STRA6 Protein (AA 1-670) (Strep Tag)

N° du produit ABIN3132014

Détail du produit

Antigène: STRA6 (Stimulated By Retinoic Acid 6 (STRA6))

Type de proteíne: Recombinant

Attributs du protein: AA 1-670

Origine: Souris

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette STRA6 protéine est marqué à la Strep Tag.

Application: Western Blotting (WB), ELISA, SDS-PAGE (SDS)

Séquence: MESQASENGS QTSSGVTDDY SSWYIEEPLG AEEVQPEGVI PLCQLTAPPA LLHACLASLS FLVLLLLALL VRRRRLWPRC GHRGLGLPSP VDFLAGDLSW TVPAAVFVVL FSNLCLLLPD ENPLPFLNLT AASSPDGEME TSRGPWKLLA LLYYPALYYP LAACASAGHQ AAFLLGTVLS WAHFGVQVWQ KAECPQDPKI YKHYSLLASL PLLLGLGFLS LWYPVQLVQS LRHRTGAGSQ GLQTSYSEKY LRTLLCPKKL DSCSHPASKR SLLSRAWAFS HHSIYTPQPG FRLPLKLVIS ATLTGTATYQ VALLLLVSVV PTVQKVRAGI NTDVSYLLAG FGIVLSEDRQ EVVELVKHHL WTVEACYISA LVLSCASTFL LLIRSLRTHR ANLQALHRGA ALDLDPPLQS IHPSRQAIVS WMSFCAYQTA FSCLGLLVQQ VIFFLGTTSL AFLVFVPLLH GRNLLLLRSL ESTWPFWLTV ALAVILQNIA ANWIFLRTHH GYPELTNRRM LCVATFLLFP INMLVGAIMA VWRVLISSLY NTVHLGQMDL SLLPQRAASL DPGYHTYQNF LRIEASQSHP GVIAFCALLL HAPSPQPRPP LAPQDSLRPA EEEEGMQLLQ TKDLMAKGAG HKGSQSRARW GLAYTLLHNP SLQAFRKAAL TSAKANGTQP
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: STRA6 (Stimulated By Retinoic Acid 6 (STRA6))

Autre désignation: Stra6 (STRA6 Produits)

Synonymes: MCOPCB8 Protein, MCOPS9 Protein, AI891933 Protein, RGD1307551 Protein, STRA6 Protein, im:7151282 Protein, wu:fc51h06 Protein, zgc:136689 Protein, mcops9 Protein, pp14296 Protein, stimulated by retinoic acid 6 Protein, stimulated by retinoic acid gene 6 Protein, stimulated by retinoic acid gene 6 homolog (mouse) Protein, STRA6 Protein, Stra6 Protein, stra6 Protein

Sujet: Receptor for retinol uptake STRA6 (Retinoic acid-responsive protein) (Retinol-binding protein receptor STRA6) (Stimulated by retinoic acid gene 6 protein),FUNCTION: Functions as a retinol transporter (PubMed:23839944, PubMed:24852372). Accepts all-trans retinol from the extracellular retinol-binding protein RBP4, facilitates retinol transport across the cell membrane, and then transfers retinol to the cytoplasmic retinol-binding protein RBP1. Retinol uptake is enhanced by LRAT, an enzyme that converts retinol to all-trans retinyl esters, the storage forms of vitamin A (By similarity). Contributes to the activation of a signaling cascade that depends on retinol transport and LRAT-dependent generation of retinol metabolites that then trigger activation of JAK2 and its target STAT5, and ultimately increase the expression of SOCS3 and inhibit cellular responses to insulin (PubMed:21368206, PubMed:23839944). Important for the homeostasis of vitamin A and its derivatives, such as retinoic acid and 11-cis-retinal (PubMed:22467576, PubMed:24852372). STRA6-mediated transport is particularly important in the eye, and under conditions of dietary vitamin A deficiency (PubMed:22467576, PubMed:23839944, PubMed:24852372). Does not transport retinoic acid (By similarity). {ECO:0000250|UniProtKB:Q9BX79, ECO:0000269|PubMed:21368206, ECO:0000269|PubMed:22467576, ECO:0000269|PubMed:23839944, ECO:0000269|PubMed:24852372}.

Poids moléculaire: 73.8 kDa

UniProt: O70491

Pathways: Feeding Behaviour