ENPEP Protein (AA 1-945) (Strep Tag)

N° du produit ABIN3133116

Détail du produit

Antigène: ENPEP (Glutamyl Aminopeptidase (Aminopeptidase A) (ENPEP))

Type de proteíne: Recombinant

Attributs du protein: AA 1-945

Origine: Souris

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette ENPEP protéine est marqué à la Strep Tag.

Application: SDS-PAGE (SDS), Western Blotting (WB), ELISA

Séquence: MNFAEEEPSK KYCIKGKHVA IICGVVVAVG LIVGLSVGLT RSCEQDTTPA PSQPPPEAST ALPPQDQNVC PDSEDESGEW KNFRLPDFIN PVHYDLEVKA LMEEDRYTGI VTISVNLSKP TRDLWLHIRE TKITKLPELR RPSGEQVPIR RCFEYKKQEY VVIQAAEDLA ATSGDSVYRL TMEFKGWLNG SLVGFYKTTY MEDGQIRSIA ATDHEPTDAR KSFPCFDEPN KKSTYSISII HPKEYSALSN MPEEKSEMVD DNWKKTTFVK SVPMSTYLVC FAVHRFTAIE RKSRSGKPLK VYVQPNQKET AEYAANITQA VFDYFEDYFA MEYALPKLDK IAIPDFGTGA MENWGLVTYR ETNLLYDPLL SASSNQQRVA SVVAHELVHQ WFGNTVTMDW WDDLWLNEGF ASFFEFLGVN HAEKDWQMLS QVLLEDVFPV QEDDSLMSSH PVVVTVSTPA EITSVFDGIS YSKGASILRM LQDWITPEKF QKGCQIYLKK FQFANAKTSD FWDSLQEASN LPVKEVMDTW TSQMGYPVVT VSGRQNITQK RFLLDSKADP SQPPSELGYT WNIPVRWADN DNSRITVYNR LDKGGITLNA NLSGDAFLKI NPDHIGFYRV NYEGGTWDWI AEALSSNHTR FSAADRSSFI DDAFALARAQ LLNYKIALNL TMYLKSEEDF LPWERVISSV SYIISMFEDD RELYPMIETY FQGQVKPVAD LLGWQDTGSH ITKLLRASIL GFACKMGDRE ALGNASQLFD SWLKGSASIP VNLRLLVYRY GMQNSGNEAA WNYTLEQYQK TSLAQEKEKL LYGLASVKDV KLLARYLEML KDPNIIKTQD VFTVIRYISY NSYGKTMAWN WIQLNWDYLV SRFTINDRYL GRIVTIAEPF NTELQLWQMQ SFFAKYPNAG AGAKPREQVL ETVKNNIEWL NVNRQSIREW FASLP
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: ENPEP (Glutamyl Aminopeptidase (Aminopeptidase A) (ENPEP))

Autre désignation: Enpep (ENPEP Produits)

Synonymes: im:7152184 Protein, si:ch211-146m5.2 Protein, 6030431M22Rik Protein, APA Protein, Bp-1/6C3 Protein, Ly-51 Protein, Ly51 Protein, CD249 Protein, gp160 Protein, glutamyl aminopeptidase Protein, ENPEP Protein, enpep Protein, ECU01_1470 Protein, pepA Protein, LOC5568916 Protein, CpipJ_CPIJ011103 Protein, Eint_011390 Protein, Halhy_0222 Protein, Enpep Protein

Sujet: Glutamyl aminopeptidase (EAP) (EC 3.4.11.7) (Aminopeptidase A) (AP-A) (BP-1/6C3 antigen) (CD antigen CD249),FUNCTION: Regulates central hypertension through its calcium-modulated preference to cleave N-terminal acidic residues from peptides such as angiotensin II. {ECO:0000250|UniProtKB:Q07075}.

Poids moléculaire: 108.0 kDa

UniProt: P16406

Pathways: Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones