ATP10D Protein (AA 1-1416) (Strep Tag)

N° du produit ABIN3136565

Détail du produit

Antigène: ATP10D (ATPase, Class V, Type 10D (ATP10D))

Type de proteíne: Recombinant

Attributs du protein: AA 1-1416

Origine: Souris

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette ATP10D protéine est marqué à la Strep Tag.

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Séquence: MTELLQWARH HWRRLSHGRA QGEDERPYNY ASLLACGGKS SRTPRPAGKH RVVIPHLQCF KDEYERFSGT YVNNRIRTTK YTLLNFVPRN LFEQFHRAAN LYFLFLVVLN WVPLVEAFQK EITMLPLVVV LTIIAIKDGL EDYRKYKIDK QINNLITKVY SRKEKKYIDC CWKNVTVGDF IRLSCNEIIP ADMVLLFSTD PDGICHIETS GLDGESNLKQ RQVVRGYTEQ DSEVDPEKFS SRIECESPNN DLSRFRGFLE HANKERVGLS KENLLLRGCT IRNTEAVVGI VVYAGHETKA MLNNSGPRYK RSKLERRANT DVLWCVLLLI VMCLTGALGH GIWLSRYENM LFFNIPEPDG RVISPVLTGF YVFWTMIILL QVLIPISLYV SIEIVKLGQI YFIQSDVDFY NEKMDSTIQC RALNITEDLG QIQYLFSDKT GTLTENKMVF RRCSVAGFDY CHEENAKRLE SYQEAVSEEE ECTDTLGGSL SNMARPRAQG CRTVPSGPLG KPSAQLSGST SAVGNGEGSG EVPHSRQAAF SSPMETDVVP DTRLLDKFSQ LTPQLLTGLD GTAQSSPLET LYIMDFFIAL AICNTVVVSA PNQPRQKIGL SSLGGMPIKS LEEIKNIFQK LSVRRSSSPS LASGKDSSSG TPCAFVSRIS FFSRPKLSPP MEDESSQMDE IPQASNSACC TETEAQNRAV GLSVSSAEAL SGPPPSASNL CYEAESPDEA ALVYAARAYR CTLQSRTPEQ VMVDFAALGS LTFQLLHILP FDSVRKRMSV VVRHPLSKQV VVYTKGADSV IMELLSVAAS DGTNPEQQMI IRERTQRHLD EYAKRGLRTL CVAKKVMSDT EYAEWLRNHF LAETSIDNRE ELLVESAMRL ENKLTLLGAT GIEDRLQEGV PESIEALHQA GIKIWMLTGD KQETAVNIAY ACKLLEPDDK LFILNTQSQD ACGMLMSAIL EELQKRAQVS PELASSRKNF PQPSDAQGQG RAGLVITGKT LEFALQESLQ RQFLELTAWC QAVICCRATP LQKSEVVKLV RNHHHVLTLP IGDGANDVSM IQVADIGIGV SGQEGMQAVM ASDFAISQFR HLSKLLLVHG HWCYTRLSNM ILYFFYKNVA YVNLLFWYQF FCGFSGTSMT DYWVLIFFNL LFTSVPPIIY GVLEKDVSAE TLLQLPELYR SGQRSEEYLP LTFWITLLDA FYQSLVCFFV PYFTYQGSDI DIFTFGNPLN TAALFIILLH LVIESKSLTW IHMLVTVGSI LSYFFFALAF GALCVTCNPP SNPYGIMRKH MLDPVFYLVC VLTTFVALLP RFLYRVLQGS VFPSPVLRAK YFDRLPPEER AEALKRWRGT AKVNHVASKH ASQSAAMSGR PTPGSSAVLA MKSATVSTVE QSTRETALDR GCSEPGASKM TGSSAS
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: ATP10D (ATPase, Class V, Type 10D (ATP10D))

Autre désignation: Atp10d (ATP10D Produits)

Synonymes: ATP10D Protein, atp10d Protein, MGC185750 Protein, ATPVD Protein, 9830145H18Rik Protein, D5Buc24e Protein, ATPase phospholipid transporting 10D (putative) Protein, ATPase, class V, type 10D Protein, ATP10D Protein, atp10d Protein, Atp10d Protein

Sujet: Phospholipid-transporting ATPase VD (EC 7.6.2.1) (ATPase class V type 10D) (P4-ATPase flippase complex alpha subunit ATP10D),FUNCTION: Catalytic component of a P4-ATPase flippase complex, which catalyzes the hydrolysis of ATP coupled to the transport of glucosylceramide (GlcCer) from the outer to the inner leaflet of the plasma membrane. {ECO:0000250|UniProtKB:Q9P241}.

Poids moléculaire: 158.3 kDa

UniProt: Q8K2X1