PCDHA10 Protein (AA 31-946) (rho-1D4 tag)

N° du produit ABIN3136824

Détail du produit

Antigène: PCDHA10 (Protocadherin alpha 10 (PCDHA10))

Type de proteíne: Recombinant

Attributs du protein: AA 31-946

Origine: Souris

Source: Cellules d'insectes

Purification/Conjugué: Cette PCDHA10 protéine est marqué à la rho-1D4 tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Séquence: QLRYSVPEEA KHGTFVGRIA QDLGLELTEL VPRLFRVASK DRGDLLEVNL QNGILFVNSR IDREELCGRS AECSIHLEVI VDRPLQVFHV EVEVRDINDN PPVFPTTEKN LFVSESRALD PHFSLEGASD ADIGTNALLT YRLSPSEYFS LEVPTTDELV KPLQLVLKKP LDRERASELH LVVKATDGGK PELTGTLELH ITVLDANDNA PAFDRAIYRV KLVENARNGT VVIRLNASDL DEGSNGQILY SFAADVSPKT EATFHIDSVS GEIKVNGKID FEETNLWKIQ AEAVDKGSPP MFGHCTILIE VLDINDNAPK IIVTSLSLPV QEDAPVGTVI ALISVMDPDS TVNGQVTCSL SPHVPFKLVS TFKNYYSLVL DSALDRETTA DYKVVVTARD GGSPALWATA SVSVEVADVN DNAPAFAQSE YTVFVKENNP PGVHVFTVLA MDADAQENAL VSYSLVERRV GERLLSSYVS VHAESGKVFA LQPLDHEELE LLQFQVTAKD AGVPALGSNV TLQVFVLDEN DNAPTLLPHG AVGAGGAVSE LVPRSVGSGH VVAKVRAVDA DSGYNAWLSY ELQFRAGSVR SPFRVGLYTG EISITRALDE ADAPRQRLLV LVKDHGEPAL TATATVLVSL VESNQAPKAS SRVLGPAASE SSVVDVNVYL IIAICAVSSL LVLTLVLYTA LRCSALPTEV TCGPGKPMLV CSSAVGSWSY SQQRRQRVCS GEGPPKTDLM AFSPSVPPGL GSGDSGVQQE IFENPRQPNP DWRYSASLRA GMHSSVHLEE AGILRAGPGG PDQQWPTVSS ATPEPEAGEV SPPVGAGVNS NSWTFKYGPG NPKQSGPGEL PDKFIIPGSP AIISIRQEPA NNQIDKSDFI TFGKKEETKK KKKKKKGNKT QEKKEKGNST TDNSDQ
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Pcdha10 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:

1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin-free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Commentaires:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: PCDHA10 (Protocadherin alpha 10 (PCDHA10))

Autre désignation: Pcdha10 (PCDHA10 Produits)

Synonymes: CNR8 Protein, CNRN8 Protein, CNRS8 Protein, CRNR8 Protein, PCDH-ALPHA10 Protein, Cnr3 Protein, Cnr8 Protein, Crnr3 Protein, Crnr8 Protein, Pcdha14 Protein, rCNRv10 Protein, protocadherin alpha 10 Protein, PCDHA10 Protein, Pcdha10 Protein

Sujet: Potential calcium-dependent cell-adhesion protein. May be involved in the establishment and maintenance of specific neuronal connections in the brain. {ECO:0000303|PubMed:11230163}.

Poids moléculaire: 99.9 kDa Including tag.

UniProt: Q91Y20