ABCB6 Protein (AA 1-842) (Strep Tag)

N° du produit ABIN3129622

Détail du produit

Antigène: ABCB6 (ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 6 (ABCB6))

Type de proteíne: Recombinant

Attributs du protein: AA 1-842

Origine: Souris

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette ABCB6 protéine est marqué à la Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Séquence: MVTVGNYCET EGPAGPAWTQ NGLSPCFFFT LVPSTLLTLG VLALVLVLPR RRREVPAGPE ELSWAAGPRV APYVLQLFLA TLQMALPLAG LAGRVGTARG VRLPGYLLLA SVLESLASVC GLWLLVVERS QARQSLAMGV WMKFRHSLGL LLLWTVTFAA ENLALVSWNS PQWWWARADL GQQVQFGLWV LRYVTSGGLF ILGLWAPGLR PQSYTLHVHE EDQDVGGNQG RSTDRRSTWR DLGRKLRLLS SYLWPRGSPS LQLIVLICLG LMGLERALNV LVPIFYRDIV NLLTAKAPWS SLAWTVTTYV FLKFLQGGGT GSTGFVSNLR TFLWIRVQQF TSRGVELRLF SHLHELSLRW HLGRRTGEVL RIVDRGTSSV TGLLSYLVFS IIPTLADIII GIIYFSMFFN AWFGLIVFLC MSLYLILTIV VTEWRAKFRR DMNTQENATR ARAVDSLLNF ETVKYYGAEG YEVDRYREAI LKFQGLEWKS TASLVVLNQT QNLVIGLGLL AGSLLCAYFV SEQKLQVGDF VLFGTYITQL YMPLNWFGTY YRMIQTNFID MENMFDLLKE ETEVKDVPGA GPLRFHKGRI EFENVHFSYA DGQETLQDVS FTVMPGQTVA LVGPSGAGKS TILRLLFRFY DISSGCIRID GQDISQVTQI SLRSHIGVVP QDTVLFNDTI ANNIRYGRVT AGDSEVEAAA QAAGIHDAIL SFPEGYETQV GERGLKLSGG EKQRVAIART ILKAPDIILL DEATSALDTS NERAIQASLA KVCTNRTTIV IAHRLSTVVN ADQILVIKDG CIIERGRHEA LLSRGGVYAE MWQLQQQGQE TVPEESKPQD TA
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: ABCB6 (ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 6 (ABCB6))

Autre désignation: Abcb6 (ABCB6 Produits)

Synonymes: ABCB6 Protein, abcb6 Protein, ABC Protein, ABC14 Protein, LAN Protein, MCOPCB7 Protein, MTABC3 Protein, PRP Protein, umat Protein, 1200005B17Rik Protein, ATP binding cassette subfamily B member 6 (Langereis blood group) Protein, ATP binding cassette subfamily B member 6 Protein, ATP-binding cassette, sub-family B (MDR/TAP), member 6a Protein, ATP-binding cassette, sub-family B (MDR/TAP), member 6 Protein, ABCB6 Protein, abcb6a Protein, abcb6 Protein, Abcb6 Protein

Sujet: ATP-binding cassette sub-family B member 6 (ABC-type heme transporter ABCB6) (EC 7.6.2.5),FUNCTION: ATP-dependent transporter that catalyzes the transport of a broad-spectrum of porphyrins from the cytoplasm to the extracellular space through the plasma membrane or into the vesicle lumen (PubMed:27507172). May also function as an ATP-dependent importer of porphyrins from the cytoplasm into the mitochondria, in turn may participate in the de novo heme biosynthesis regulation and in the coordination of heme and iron homeostasis during phenylhydrazine stress (PubMed:22294697, PubMed:17006453). May play a key role in the early steps of melanogenesis producing PMEL amyloid fibrils (By similarity). In vitro, it confers to cells a resistance to toxic metal such as arsenic and cadmium and against chemotherapeutics agent such as 5-fluorouracil, SN-38 and vincristin (By similarity). In addition may play a role in the transition metal homeostasis (By similarity). {ECO:0000250|UniProtKB:O70595, ECO:0000250|UniProtKB:Q9NP58, ECO:0000269|PubMed:17006453, ECO:0000269|PubMed:22294697, ECO:0000269|PubMed:27507172}.

Poids moléculaire: 93.8 kDa

UniProt: Q9DC29

Pathways: Transition Metal Ion Homeostasis