SLCO1C1 Protein (AA 1-715) (Strep Tag)

N° du produit ABIN3137208

Détail du produit

Antigène: SLCO1C1 (Solute Carrier Organic Anion Transporter Family, Member 1C1 (SLCO1C1))

Type de proteíne: Recombinant

Attributs du protein: AA 1-715

Origine: Souris

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette SLCO1C1 protéine est marqué à la Strep Tag.

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Séquence: MDTSSKENAH LFHKNSAQPA GGPSFTVGYP STEEARPCCG KLKVFLGALS FVYFAKALAE GYLKSTVTQI ERRFEIPSSL VGIIDGSFEI GNLLVITFVS YFGAKLHRPK IIGAGCLVMG FGTMLIAVPQ FFMEKYSYEK YERYSPSSNV TPSISPCYLE SSSPSPSSIL GKSQNKISHE CVGDSSSSMW VYVFLGNLLR GLGETPIQPL GIAYLDDFAS EDNAAFYIGC VQTVAIIGPI FGFLLGSLCA KLYVDIGFVN LDHITITPKD PQWVGAWWLG YLIAGFLSLL AAVPFWCLPK TLPRSQSREN SGSTSEKSKF IDDPIHYQMA PGDDKMKIME MAKDFLPSLK TLFRNPVYIL YLCASTVQFN SLFGMVTYKP KYIEQQYGQS SSKANFVIGL INIPAVALGI FSGGIVMKKF RLGICEATKL YLGSSVFGYL LFLSLFALGC ENSSVAGLTV SYQGTKPVSY HERALFSDCN SRCKCSDSKW EPMCGDNGIT YVSACLAGCQ SSSRSGKNII FSNCTCVGFA APKSGNWSGM MGRCQKDNGC SQMFLYFLVI SVITSYTLSL GGIPGYILLL RCIQPQLKSF ALGIYTLAVR VLAGIPAPVY FGVLIDTSCL KWGFKKCGSR GSCRLYDSHA FRHIYLGLTT LLGTVSVFLS MAVLFVLKKK YVSKHSSLIT TREKIGMSSS IKKETCAARD RGLQPKYWPG KETRL
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: SLCO1C1 (Solute Carrier Organic Anion Transporter Family, Member 1C1 (SLCO1C1))

Autre désignation: Slco1c1 (SLCO1C1 Produits)

Synonymes: OATP-F Protein, OATP1 Protein, OATP14 Protein, OATP1C1 Protein, OATPF Protein, OATPRP5 Protein, SLC21A14 Protein, OATP-14 Protein, Oatp2 Protein, Oatpf Protein, Slc21a14 Protein, Bsat1 Protein, Oatp14 Protein, solute carrier organic anion transporter family member 1C1 Protein, solute carrier organic anion transporter family, member 1c1 Protein, SLCO1C1 Protein, Slco1c1 Protein

Sujet: Solute carrier organic anion transporter family member 1C1 (Organic anion transporter 2) (OATP2) (Organic anion transporter F) (OATP-F) (Organic anion-transporting polypeptide 14) (OATP-14) (Solute carrier family 21 member 14) (Thyroxine transporter),FUNCTION: Mediates the Na(+)-independent high affinity transport of thyroid hormones at the plasma membrane of brain capillary endothelial cells (PubMed:15166123, PubMed:24691440, PubMed:22294745). The transport activity of substrates L-thyroxine (T4) and 3,3',5'-triiodo-L-thyronine (reverse T3, rT3) is much greater than that of 3,3',5-triiodo-L-thyronine (T3) (PubMed:15166123). The prehormone, T4, is the major form in the circulating blood and is converted to the active form, T3, by the iodothyronine-deiodinase in peripheral organs (PubMed:15166123). T3 plays an essential role in brain development via binding to specific nuclear receptors (thyroid hormone receptor) (PubMed:15166123, PubMed:22294745). Also transports organic anions such as the conjugated steroid 17-beta-glucuronosyl estradiol (17beta-estradiol 17-O-(beta-D-glucuronate)) (PubMed:15166123). Transports T4 and estrone-3-sulfate in a pH -insensitive manner (By similarity). May serve as a drug efflux system at the blood brain barrier (By similarity). {ECO:0000250|UniProtKB:Q9EPZ7, ECO:0000250|UniProtKB:Q9NYB5, ECO:0000269|PubMed:15166123, ECO:0000269|PubMed:22294745, ECO:0000269|PubMed:24691440, ECO:0000303|PubMed:15166123, ECO:0000303|PubMed:22294745}.

Poids moléculaire: 78.3 kDa

UniProt: Q9ERB5