ABCB11 Protein (AA 1-1321) (Strep Tag)

N° du produit ABIN3137386

Détail du produit

Antigène: ABCB11 (ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 11 (ABCB11))

Type de proteíne: Recombinant

Attributs du protein: AA 1-1321

Origine: Souris

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette ABCB11 protéine est marqué à la Strep Tag.

Application: SDS-PAGE (SDS), Western Blotting (WB), ELISA

Séquence: MSDSVILRSV KKFGEENHAF ESDGFHNNDK KSRLQDKKKG EGARVGFFEL FRFSSSKDNW LMFMGSVCAL LHGMAQPGMI IVFGILTDIF VEYDIERQEL SIPEKVCMNN TIVWINSSFN QNMTNGTSCG LVDINSEVIK FSGIYAGVGV AVLILGYFQI RLWVITGARQ IRKMRKFYFR RIMRMEIGWF DCTSVGELNS RFSDDINKID EAIADQMALF LQRLSTALSG LLLGFYRGWK LTLVILAVSP LIGIGAAVIG LSVAKFTELE LKAYAKAGSI ADEVLSSIRT VAAFGGENKE VERYEKNLMF AQRWGIWKGM VMGFFTGYMW CLIFFCYALA FWYGSRLVLD EGEYTPGTLI QIFLCVIIAA MNIGNASSCL EIFSTGCSAA SSIFQTIDRQ PVMDCMSGDG YKLDRIKGEI EFHNVTFHYP SRPEVKILNN LSMVIKPGET TAFVGSSGAG KSTALQLIQR FYDPCEGMVT LDGHDIRSLN IRWLRDQIGI VEQEPVLFST TIAENIRLGR EEATMEDIVQ AAKDANAYNF IMALPQQFDT LVGEGGGQMS GGQKQRVAIA RALIRKPKIL LLDMATSALD NESEAKVQGA LNKIQHGHTI ISVAHRLSTV RSADVIIGFE HGTAVERGTH EELLERKGVY FMLVTLQSQE DNTHKETGIK GKDTTEGDTP ERTFSRGSYQ DSLRASIRQR SKSQLSHLSH EPPLAIGDHK SSYEDRKDND VLVEEVEPAP VRRILKYNIS EWPYILVGAL CAAINGAVTP IYSLLFSQIL KTFSLVDKEQ QRSEIYSMCL FFVILGCVSL FTQFLQGYNF AKSGELLTKR LRKFGFKAML RQDIGWFDDL KNNPGVLTTR LATDASQVQG ATGSQVGMMV NSFTNIFVAV LIAFLFNWKL SLVISVFFPF LALSGAVQTK MLTGFASQDK EILEKAGQIT NEALSNIRTV AGIGVEGRFI KAFEVELEKS YKTAIRKANV YGLCYAFSQG ISFLANSAAY RYGGYLIVYE DLNFSYVFRV VSSIAMSATA VGRTFSYTPS YAKAKISAAR FFQLLDRKPP IDVYSGAGEK WDNFQGKIDF IDCKFTYPSR PDIQVLNGLS VSVDPGQTLA FVGSSGCGKS TSIQLLERFY DPDQGTVMID GHDSKKVNVQ FLRSNIGIVS QEPVLFDCSI MDNIKYGDNT KEISVERAIA AAKQAQLHDF VMSLPEKYET NVGIQGSQLS RGEKQRIAIA RAIVRDPKIL LLDEATSALD TESEKTVQLA LDKAREGRTC IVIAHRLSTI QNSDIIAVMS QGVVIEKGTH KKLMDQKGAY YKLVITGAPI S
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: ABCB11 (ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 11 (ABCB11))

Autre désignation: Abcb11 (ABCB11 Produits)

Synonymes: ABCB11 Protein, Bsep Protein, ABC16 Protein, BRIC2 Protein, BSEP Protein, PFIC-2 Protein, PFIC2 Protein, PGY4 Protein, SPGP Protein, Lith1 Protein, BSEP/SPGP Protein, Spgp Protein, ATP binding cassette subfamily B member 11 Protein, bile salt export pump Protein, ATP-binding cassette, sub-family B (MDR/TAP), member 11 Protein, ABCB11 Protein, LOC100489744 Protein, LOC100565276 Protein, EDI_111220 Protein, EDI_272930 Protein, abcb11 Protein, Abcb11 Protein

Sujet: Bile salt export pump (EC 7.6.2.-) (ATP-binding cassette sub-family B member 11) (Sister of P-glycoprotein),FUNCTION: Catalyzes the transport of the major hydrophobic bile salts, such as taurine and glycine-conjugated cholic acid across the canalicular membrane of hepatocytes in an ATP-dependent manner, therefore participates in hepatic bile acid homeostasis and consequently to lipid homeostasis through regulation of biliary lipid secretion in a bile salts dependent manner (PubMed:14570929, PubMed:11172067, PubMed:23764895, PubMed:22619174, PubMed:19228692). Transports taurine-conjugated bile salts more rapidly than glycine-conjugated bile salts (By similarity). Also transports non-bile acid compounds, such as pravastatin and fexofenadine in an ATP-dependent manner and may be involved in their biliary excretion (By similarity). {ECO:0000250|UniProtKB:O95342, ECO:0000269|PubMed:11172067, ECO:0000269|PubMed:14570929, ECO:0000269|PubMed:19228692, ECO:0000269|PubMed:22619174, ECO:0000269|PubMed:23764895}.

Poids moléculaire: 146.7 kDa

UniProt: Q9QY30