IL-3 Protéine

N° du produit ABIN5505701

Protéine Recombinant IL-3 exprimée dans Cellules d'insectes.

Aperçu rapide pour IL-3 Protéine (ABIN5505701)

Antigène: IL-3 (Interleukin 3 (IL-3))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: Cellules d'insectes

Application: Functional Studies (Func), Antibody Production (AbP), Protein Interaction (PI), Standard (STD)

Pureté: > 98 % , as determined by Coomassie stained SDS-PAGE.

Détail du produit

Specificité: Optimal preservation of protein structure, post-translational modifications and functions.

Attributs du produit:

• Recombinant human Interleukin-3 / IL3 protein expressed in Insect cells.
• Produced with end-sequenced ORF clone
• Tested for bioactivity.

niveau d'endotoxine: Less than 0.01 ng per μg protein as determined by the LAL method.

Biological Activity Comment: The ED50 is 0.10 - 0.30 ng/ml, corresponding to a specific activity of 0.33 - 1.0 x 10^7 units/mg, determined by the dose dependent stimulation of TF-1 cell proliferation assay. Under this assay the bioactivity is equivalent to competit

Information d'application

Indications d'application: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays

Restrictions: For Research Use only

Stockage

Concentration: > 50 μg/mL

Buffer: 20 mM NaH2PO4, 0.30 M NaCl, pH 6.0

Stock: -80 °C

Stockage commentaire: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Détail du antigène

Antigène: IL-3 (Interleukin 3 (IL-3))

Autre désignation: Interleukin-3 / IL3

Sujet: The protein encoded by this gene is a potent growth promoting cytokine. This cytokine is capable of supporting the proliferation of a broad range of hematopoietic cell types. It is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. This cytokine has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders.

Poids moléculaire: 15 kDa

NCBI Accession: NP_000579

Pathways: Signalistation JAK/STAT, Regulation of Carbohydrate Metabolic Process, Autophagy