Lectin, Galactoside-Binding, Soluble, 9 (LGALS9) (AA 1-355) (Active) protein (His tag,GST tag)

Détails pour le produit réf. ABIN5665803, Fournisseur: Connectez-vous pour afficher
Nom de la protéine
  • huat
  • lgals9a
  • ecalectin
  • galectin-9
  • Lgals9
  • wu:fd20c09
  • zgc:111833
  • LGALS9
  • AA407335
  • AI194909
  • AI265545
  • LGALS35
  • Lgals5
  • gal-9
  • HUAT
  • LGALS9A
  • UAT
  • UATP.I
  • galectin 9 L homeolog
  • galectin 9
  • lectin, galactoside-binding, soluble, 9 (galectin 9)-like 3
  • lectin, galactose binding, soluble 9
  • lgals9.L
  • lgals9
  • lgals9l3
  • LGALS9
  • Lgals9
Attributs du protein
AA 1-355
5
4
4
3
3
2
2
1
1
1
1
1
1
1
1
Origine
Humain
22
10
5
3
1
1
Source
Escherichia coli (E. coli)
26
7
2
2
2
1
1
Activité biologique
Active
Purification/Conjugué
His tag,GST tag
Application
Activity Assay (AcA), Cell Culture (CC)
Options
Fournisseur
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N° du produit (Fournisseur)
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Attributs du produit Tag location: N-terminal His and GST Tag
Pureté > 90 %
Biological Activity Comment GAL9 (Galectin-9) belongs to the galectin family, which is defined by their binding specificity for β-galactoside sugars, such as N-acetyllactosamine (Galβ1-3GlcNAc or Galβ1-4GlcNAc). It is reported that GAL9 induces T-helper type 1 lymphocyte (Th1) death by binding to HAVCR2 (Hepatitis A virus cellular receptor 2); besides, the interaction between GAL9 and PDI (Protein disulfide-isomerase) leads to disulfide reductase activity increasing at the plasma membrane, therefore alters the plasma membrane redox state and enhances cell migration. Thus a binding ELISA assay was conducted to detect the interaction of recombinant human GAL9 with recombinant human HAVCR2 and recombinant human PDI separately. Briefly, GAL9 were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to HAVCR2-coated and PDI-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-GAL9 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of GAL9 with HAVCR2 and PDI were shown in Figure 1 and Figure 2, and this effect was in a dose dependent manner The binding activity of GAL9 with HAVCR2. Figure 2. The binding activity of GAL9 with PDI.
Nom de la protéine
Sujet Alternative Names: LGALS9, Ecalectin, Lectin,Galactoside-Binding Soluble 9, Tumor antigen HOM-HD-21
Poids moléculaire 69kDa
UniProt O00182
Indications d'application Isoelectric Point: 9.3
Restrictions For Research Use only
Format Lyophilized
Buffer 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
Agent conservateur Dithiothreitol (DTT), Other preservative, ProClin
Précaution d'utilisation This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
Images (Fournisseur)
 image for Lectin, Galactoside-Binding, Soluble, 9 (LGALS9) (AA 1-355) (Active) protein (His tag,GST tag) (ABIN5665803) GAL9 (Galectin-9) belongs to the galectin family, which is defined by their binding s...
 image for Lectin, Galactoside-Binding, Soluble, 9 (LGALS9) (AA 1-355) (Active) protein (His tag,GST tag) (ABIN5665803) Lectin, Galactoside-Binding, Soluble, 9 (LGALS9) (AA 1-355) (Active) protein (His tag,GST tag) (Image 2)
 image for Lectin, Galactoside-Binding, Soluble, 9 (LGALS9) (AA 1-355) (Active) protein (His tag,GST tag) (ABIN5665803) Lectin, Galactoside-Binding, Soluble, 9 (LGALS9) (AA 1-355) (Active) protein (His tag,GST tag) (Image 3)
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