Interleukin 24 (IL24) (AA 51-206) (Active) protein (His tag)

Détails pour le produit réf. ABIN5665855, Fournisseur: Connectez-vous pour afficher
Nom de la protéine
  • C49A
  • FISP
  • IL10B
  • MDA7
  • MOB5
  • ST16
  • Mda7
  • St16
  • Mda-7
  • IL24
  • interleukin 24
  • IL24
  • Il24
Attributs du protein
AA 51-206
2
2
2
2
1
1
1
1
1
1
1
Origine
Humain
19
3
3
Source
Escherichia coli (E. coli)
8
5
3
2
2
1
1
1
1
Activité biologique
Active
Purification/Conjugué
His tag
Application
Activity Assay (AcA), Cell Culture (CC)
Options
Fournisseur
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N° du produit (Fournisseur)
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Attributs du produit Tag location: N-terminal His Tag
Pureté > 90 %
Biological Activity Comment IL24 (interleukin 24) is a cytokine that belongs to IL10 family. This protein can induce apoptosis selectively in various cancer cells, including ECV304. Thus, inhibition of cell proliferation assay of IL24 was conducted using ECV-304 cells. Briefly, ECV-304 cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard 1640 prior to the addition of various concentrations of IL24. After incubated for 48h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10μL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37°C. Inhibition of ECV-304 cells proliferation after incubation with IIL24 for 48h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with various concentrations of IL24 for 48h. The mean OD value of ECV-304 assessed by CCK-8 was shown in Figure 2. It was obvious that IL24 significantly decreased cell viability of ECV-304 cells.
Nom de la protéine
Sujet Alternative Names: C49A, FISP, IL10B, MDA7, Mob-5, ST16, Mda-7, Melanoma Differentiation Association Protein 7, Suppression Of Tumorigenicity 16
Poids moléculaire 18kDa
UniProt Q13007
Pathways Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity, Autophagy
Indications d'application Isoelectric Point: 8.6
Restrictions For Research Use only
Format Lyophilized
Buffer 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
Agent conservateur Dithiothreitol (DTT), Other preservative, ProClin
Précaution d'utilisation This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
Images (Fournisseur)
SDS-PAGE (SDS) image for Interleukin 24 (IL24) (AA 51-206) (Active) protein (His tag) (ABIN5665855) Figure. SDS-PAGE; Sample: Active recombinant IL24, Human.
 image for Interleukin 24 (IL24) (AA 51-206) (Active) protein (His tag) (ABIN5665855) IL24 (interleukin 24) is a cytokine that belongs to IL10 family. This protein can ind...
 image for Interleukin 24 (IL24) (AA 51-206) (Active) protein (His tag) (ABIN5665855) Interleukin 24 (IL24) (AA 51-206) (Active) protein (His tag) (Image 3)