PHD1 Protein (AA 283-407, partial) (His tag)

N° du produit ABIN5709449

Détail du produit

Antigène: PHD1 (EGLN2) (Egl-9 Family Hypoxia Inducible Factor 2 (EGLN2))

Type de proteíne: Recombinant

Attributs du protein: AA 283-407, partial

Origine: Humain

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette PHD1 protéine est marqué à la His tag.

Application: SDS-PAGE (SDS)

Séquence: MVACYPGNGL GYVRHVDNPH GDGRCITCIY YLNQNWDVKV HGGLLQIFPE GRPVVANIEP LFDRLLIFWS DRRNPHEVKP AYATRYAITV WYFDAKERAA AKDKYQLASG QKGVQVPVSQ PPTPT

Purification: SDS-PAGE

Pureté: > 90 %

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.1-2 mg/mL

Buffer: 20 mM Tris-HCl based buffer, pH 8.0

Stock: -80 °C,4 °C,-20 °C

Stockage commentaire: Store at -20°C, for extended storage, conserve at -20°C or -80°C. Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Détail du antigène

Antigène: PHD1 (EGLN2) (Egl-9 Family Hypoxia Inducible Factor 2 (EGLN2))

Autre désignation: EGLN2 (EGLN2 Produits)

Synonymes: egln2 Protein, phd2 Protein, PHD1 Protein, 0610011A13Rik Protein, C85656 Protein, Hif-p4h-1 Protein, Ier4 Protein, Phd1 Protein, SM-20 Protein, HIF-PH1 Protein, HPH-1 Protein, HPH-3 Protein, PHD-1 Protein, EIT6 Protein, HIFPH1 Protein, egl-9 family hypoxia-inducible factor 1 Protein, egl-9 family hypoxia inducible factor 2 Protein, Egl nine homolog 2 Protein, egl-9 family hypoxia-inducible factor 2 L homeolog Protein, egl-9 family hypoxia-inducible factor 2 Protein, egln1 Protein, EGLN2 Protein, egln2 Protein, egln2.L Protein, Egln2 Protein

Sujet: Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. Also hydroxylates HIF2A. Has a preference for the CODD site for both HIF1A and HIF2A. Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Under hypoxic conditions, the hydroxylation reaction is attenuated allowing HIFs to escape degradation resulting in their translocation to the nucleus, heterodimerization with HIF1B, and increased expression of hypoxy-inducible genes. EGLN2 is involved in regulating hypoxia tolerance and apoptosis in cardiac and skeletal muscle. Also regulates susceptibility to normoxic oxidative neuronal death. Links oxygen sensing to cell cycle and primary cilia formation by hydroxylating the critical centrosome component CEP192 which promotes its ubiquitination and subsequent proteasomal degradation. Hydroxylates IKBKB, mediating NF-kappaB activation in hypoxic conditions. Target proteins are preferencially recognized via a LXXLAP motif.

Poids moléculaire: 18.2 kDa

UniProt: Q96KS0

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Cell RedoxHomeostasis