UBE2W Protein (AA 1-151, full length) (His-SUMO Tag)

N° du produit ABIN5711601

Détail du produit

Antigène: UBE2W (Ubiquitin-Conjugating Enzyme E2W (UBE2W))

Type de proteíne: Recombinant

Attributs du protein: full length, AA 1-151

Origine: Humain

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette UBE2W protéine est marqué à la His-SUMO Tag.

Application: SDS-PAGE (SDS)

Séquence: MASMQKRLQK ELLALQNDPP PGMTLNEKSV QNSITQWIVD MEGAPGTLYE GEKFQLLFKF SSRYPFDSPQ VMFTGENIPV HPHVYSNGHI CLSILTEDWS PALSVQSVCL SIISMLSSCK EKRRPPDNSF YVRTCNKNPK KTKWWYHDDT C

Purification: SDS-PAGE

Pureté: > 90 %

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.1-2 mg/mL

Buffer: 20 mM Tris-HCl based buffer, pH 8.0

Stock: -80 °C,4 °C,-20 °C

Stockage commentaire: Store at -20°C, for extended storage, conserve at -20°C or -80°C. Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Détail du antigène

Antigène: UBE2W (Ubiquitin-Conjugating Enzyme E2W (UBE2W))

Autre désignation: UBE2W (UBE2W Produits)

Synonymes: UBE2W Protein, im:6905459 Protein, si:ch211-193n21.4 Protein, ube2wa Protein, UBC-16 Protein, UBC16 Protein, 6130401J04Rik Protein, ubiquitin conjugating enzyme E2 W Protein, ubiquitin-conjugating enzyme E2W (putative) Protein, ubiquitin conjugating enzyme E2 W (putative) Protein, ubiquitin conjugating enzyme E2W (putative) Protein, ubiquitin-conjugating enzyme E2W Protein, UBE2W Protein, ube2w Protein, Ube2w Protein

Sujet: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes monoubiquitination. Involved in degradation of misfolded chaperone substrates by mediating monoubiquitination of STUB1/CHIP, leading to recruitment of ATXN3 to monoubiquitinated STUB1/CHIP, and restriction of the length of ubiquitin chain attached to STUB1/CHIP substrates by ATXN3. After UV irradiation, but not after mitomycin-C (MMC) treatment, acts as a specific E2 ubiquitin-conjugating enzyme for the Fanconi ania complex by associating with E3 ubiquitin-protein ligase FANCL and catalyzing monoubiquitination of FANCD2, a key step in the DNA damage pathway. In vitro catalyzes 'Lys-11'-linked polyubiquitination. Transfers ubiquitin in complex with RING/U-box type E3s that do not have active site cysteine residues to form thioester bonds with ubiquitin, and preferentially ubiquitinates the N-terminus of substrates, such as ATXN3, STUB1 and SUMO2.

Poids moléculaire: 33.3 kDa

UniProt: Q96B02