SLC26A5 Protein (AA 501-744) (His tag)

N° du produit ABIN5713838

Détail du produit

Antigène: SLC26A5 (Solute Carrier Family 26, Member 5 (Prestin) (SLC26A5))

Type de proteíne: Recombinant

Attributs du protein: AA 501-744

Origine: Humain

Source: Levure

Purification/Conjugué: Cette SLC26A5 protéine est marqué à la His tag.

Application: SDS-PAGE (SDS)

Séquence: YRTQSPSYKV LGKLPETDVY IDIDAYEEVK EIPGIKIFQI NAPIYYANSD LYSNALKRKT GVNPAVIMGA RRKAMRKYAK EVGNANMANA TVVKADAEVD GEDATKPEEE DGEVKYPPIV IKSTFPEEMQ RFMPPGDNVH TVILDFTQVN FIDSVGVKTL AGIVKEYGDV GIYVYLAGCS AQVVNDLTRN RFFENPALWE LLFHSIHDAV LGSQLREALA EQEASAPPSQ EDLEPNATPA TPEA

Purification: SDS-PAGE

Pureté: > 90 %

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.1-2 mg/mL

Buffer: 20 mM Tris-HCl based buffer, pH 8.0

Stock: -80 °C,4 °C,-20 °C

Stockage commentaire: Store at -20°C, for extended storage, conserve at -20°C or -80°C. Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Détail du antigène

Antigène: SLC26A5 (Solute Carrier Family 26, Member 5 (Prestin) (SLC26A5))

Autre désignation: S26A5 (SLC26A5 Produits)

Synonymes: pres Protein, fb73d12 Protein, fb74g12 Protein, wu:fb73d12 Protein, wu:fb74g12 Protein, DFNB61 Protein, PRES Protein, Pres Protein, prestin Protein, solute carrier family 26 (anion exchanger), member 5 Protein, solute carrier family 26 member 5 Protein, solute carrier family 26, member 5 Protein, slc26a5 Protein, SLC26A5 Protein, Slc26a5 Protein

Sujet: Motor protein that converts auditory stimuli to length changes in outer hair cells and mediates sound amplification in the mammalian hearing organ. Prestin is a bidirectional voltage-to-force converter, it can operate at microsecond rates. It uses cytoplasmic anions as extrinsic voltage sensors, probably chloride and bicarbonate. After binding to a site with millimolar affinity, these anions are translocated across the mbrane in response to changes in the transmbrane voltage. They move towards the extracellular surface following hyperpolarization, and towards the cytoplasmic side in response to depolarization. As a consequence, this translocation triggers conformational changes in the protein that ultimately alter its surface area in the plane of the plasma mbrane. The area decreases when the anion is near the cytoplasmic face of the mbrane (short state), and increases when the ion has crossed the mbrane to the outer surface (long state). So, it acts as an incomplete transporter. It swings anions across the mbrane, but does not allow these anions to dissociate and escape to the extracellular space. Salicylate, an inhibitor of outer hair cell motility, acts as competitive antagonist at the prestin anion-binding site .

Poids moléculaire: 28.96 kDa

UniProt: P58743

Pathways: Sensory Perception of Sound, Dicarboxylic Acid Transport