TFPI2 Protein (His tag)

N° du produit ABIN573781

Détail du produit

Antigène: TFPI2 (Tissue Factor Pathway Inhibitor 2 (TFPI2))

Type de proteíne: Recombinant

Origine: Humain

Source: Tobacco (Nicotiana benthamiana)

Purification/Conjugué: Cette TFPI2 protéine est marqué à la His tag.

Application: Functional Studies (Func)

Séquence: HHHHHHGAAQ EPTGNNAEIC LLPLDYGPCK ALLLRYYYDR YTQSCRQFLY GGCEGNANNF YTWEACDDAC WRIEKVPKV

Attributs du produit: Molecular formula: C407H592N116O117S6.
Isoelectric Point: 6,25.
Extinction coefficient: E 0.1 % (1g/L) = 2.35 (A 280 nm).
This product contains no animal-derived components or impurities. It is produced by transient expression in non-transgenic plants.

Purification: Recombinant AGV212 includes a 6 His-tag at the N-terminal end, is purified by sequential chromatography (FPLC).

Pureté: > 97 % by SDS polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.

niveau d'endotoxine: < 0.04 EU/µg protein (LAL method)

Information d'application

Indications d'application: The ability to prevent the hydrolysis of benzoyl-Larginine ethyl ester hydrochloride by trypsin.

Commentaires:

Biological Activity: The activity of the inhibitor is expressed as the amount of trypsin inhibited per milligram of inhibitor. The ability to prevent the hydrolysis of benzoyl-L-arginine ethyl ester hydrochloride by trypsin is measured by spectrophotometer. One mg protein will inhibit 1-1.5 mg trypsin with activity of approximately 1000 BAEE units per mg protein.
Synonyms: First Kunitz domain of the human Tissue Factor Protein Inhibitor 2 (TFPI-2) protein, AGV 212

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Lyophilized protein should be reconstituted adding 1 mL of sterile water to the vial, which gives a concentration of 1 mg of protease inhibitor per mL. At higher concentrations the solubility may be reduced and multimers generated. Optimal concentration should be determined for specific application and cell lines.

Concentration: 50 ng/µL

Buffer: PBS, pH 7.1.

Stock: 4 °C

Détail du antigène

Antigène: TFPI2 (Tissue Factor Pathway Inhibitor 2 (TFPI2))

Autre désignation: TFPI-2 (TFPI2 Produits)

Synonymes: MGC68843 Protein, TFPI2 Protein, MGC108301 Protein, si:ch211-262k23.2 Protein, tfpi2 Protein, PP5 Protein, REF1 Protein, TFPI-2 Protein, AV000670 Protein, PP5/TFPI-2 Protein, tissue factor pathway inhibitor 2 L homeolog Protein, tissue factor pathway inhibitor 2 Protein, tfpi2.L Protein, TFPI2 Protein, tfpi2 Protein, Tfpi2 Protein

Sujet: Recombinant human TFPI-2 doman 1 or AGV 212 is a protease inhibitor peptide generated from the first Kunitz domain of the human Tissue Factor Protein Inhibitor 2 (TFPI-2) protein, after site-directed mutagenesis to increase its activity. It is arranged in a single polypeptide chain that is linked by three disulfide bridges. AGV 212 is quite stable and inhibits trypsin with high efficiency and Ki lower than TFPI-2 one. TFPI-2 has been shown to inhibit Endothelial Cell Matrix (ECM) proteases essential for angiogenesis and metastasis.

Poids moléculaire: 9.3 kDa