IMPACT Protein (AA 1-320) (His tag)
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- Antigène Voir toutes IMPACT Protéines
- IMPACT (Impact Homolog (IMPACT))
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 1-320
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Origine
- Humain
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Source
- Escherichia coli (E. coli)
- Purification/Conjugué
- Cette IMPACT protéine est marqué à la His tag.
- Application
- SDS-PAGE (SDS)
- Séquence
- MGSSHHHHHH SSGLVPRGSH MGSMAEGDAG SDQRQNEEIE AMAAIYGEEW CVIDDCAKIF CIRISDDIDD PKWTLCLQVM LPNEYPGTAP PIYQLNAPWL KGQERADLSN SLEEIYIQNI GESILYLWVE KIRDVLIQKS QMTEPGPDVK KKTEEEDVEC EDDLILACQP ESSVKALDFD ISETRTEVEV EELPPIDHGI PITDRRSTFQ AHLAPVVCPK QVKMVLSKLY ENKKIASATH NIYAYRIYCE DKQTFLQDCE DDGETAAGGR LLHLMEILNV KNVMVVVSRW YGGILLGPDR FKHINNCARN ILVEKNYTNS PEESSKALGK NKKVRKDKKR NEH
- Pureté
- > 90 % by SDS - PAGE
- Top Product
- Discover our top product IMPACT Protéine
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Impact Homolog (IMPACT) (AA 1-320) protein (His tag)
IMPACT Origine: Humain Hôte: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Impact Homolog (IMPACT) (AA 1-320) protein (GST tag)IMPACT Origine: Humain Hôte: Wheat germ Recombinant ELISA, WB, AP, AA
Impact Homolog (IMPACT) (AA 1-320) protein (Strep Tag)Crystallography grade IMPACT Origine: Humain Hôte: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
Impact Homolog (IMPACT) (AA 1-318) protein (Strep Tag)Crystallography grade IMPACT Origine: Souris Hôte: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
Impact Homolog (IMPACT) protein (Myc-DYKDDDDK Tag)IMPACT Origine: Humain Hôte: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Indications d'application
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1.0 mg/mL
- Buffer
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 0.15M NaCl, 10 % glycerol, 1 mM DTT
- Stock
- 4 °C,-20 °C,-80 °C
- Stockage commentaire
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Antigène
- IMPACT (Impact Homolog (IMPACT))
- Autre désignation
- IMPACT (IMPACT Produits)
- Synonymes
- zgc:103672 Protein, Ximpact Protein, impact-A Protein, RWDD5 Protein, impact-a Protein, E430016J11Rik Protein, impact RWD domain protein Protein, impact Protein, impact RWD domain protein L homeolog Protein, impact, RWD domain protein Protein, IMPACT Protein, impact Protein, EDI_298310 Protein, impact.L Protein, Impact Protein
- Sujet
- IMPACT belongs to the IMPACT family and contains 1 RWD domain. This protein is translational regulator that ensures constant high levels of translation under amino acid starvation and acts by interacting with GCN1/GCN1L1, thereby preventing activation of GCN2 protein kinases (EIF2AK1 to 4) and subsequent down-regulation of protein synthesis. Recombinant human IMPACT protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Poids moléculaire
- 38.9kDa (343aa), confirmed by MALDI-TOF
- NCBI Accession
- NP_060909
- UniProt
- Q9P2X3
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