RNGTT Protein (AA 1-597) (His tag)

N° du produit ABIN5853868

Détail du produit

Antigène: RNGTT (RNA Guanylyltransferase and 5'-Phosphatase (RNGTT))

Type de proteíne: Recombinant

Attributs du protein: AA 1-597

Origine: Humain

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette RNGTT protéine est marqué à la His tag.

Application: SDS-PAGE (SDS)

Séquence: MGSSHHHHHH SSGLVPRGSH MGSMAHNKIP PRWLNCPRRG QPVAGRFLPL KTMLGPRYDS QVAEENRFHP SMLSNYLKSL KVKMGLLVDL TNTSRFYDRN DIEKEGIKYI KLQCKGHGEC PTTENTETFI RLCERFNERN PPELIGVHCT HGFNRTGFLI CAFLVEKMDW SIEAAVATFA QARPPGIYKG DYLKELFRRY GDIEEAPPPP LLPDWCFEDD EDEDEDEDGK KESEPGSSAS FGKRRKERLK LGAIFLEGVT VKGVTQVTTQ PKLGEVQQKC HQFCGWEGSG FPGAQPVSMD KQNIKLLDLK PYKVSWKADG TRYMMLIDGT NEVFMIDRDN SVFHVSNLEF PFRKDLRMHL SNTLLDGEMI IDRVNGQAVP RYLIYDIIKF NSQPVGDCDF NVRLQCIERE IISPRHEKMK TGLIDKTQEP FSVRNKPFFD ICTSRKLLEG NFAKEVSHEM DGLIFQPTGK YKPGRCDDIL KWKPPSLNSV DFRLKITRMG GEGLLPQNVG LLYVGGYERP FAQIKVTKEL KQYDNKIIEC KFENNSWVFM RQRTDKSFPN AYNTAMAVCN SISNPVTKEM LFEFIDRCTA ASQGQKRKHH LDPDTELMPP PPPKRPRPLT

Pureté: > 85 % by SDS - PAGE

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: Liquid. In 20  mM Tris-HCl buffer (  pH 7.5) containing 0.2M NaCl, 40 % glycerol, 2  mM DTT, 0.1  mM PMSF

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Détail du antigène

Antigène: RNGTT (RNA Guanylyltransferase and 5'-Phosphatase (RNGTT))

Autre désignation: RNGTT (RNGTT Produits)

Synonymes: xcap1b Protein, MGC75787 Protein, CAP1A Protein, HCE Protein, HCE1 Protein, hCAP Protein, AU020997 Protein, zgc:76886 Protein, RNA guanylyltransferase and 5'-phosphatase Protein, mRNA-capping enzyme Protein, rngtt.S Protein, rngtt Protein, RNGTT Protein, LOC100439097 Protein, Rngtt Protein

Sujet: RNGTT is a bifunctional mRNA-capping enzyme exhibiting RNA 5'-triphosphatase activity in the N-terminal part and mRNA guanylyltransferase activity in the C-terminal part. It catalyzes the first two steps of cap formation: by removing the gamma-phosphate from the 5'-triphosphate end of nascent mRNA to yield a diphosphate end, and by transferring the gmp moiety of GTP to the 5'-diphosphate terminus. Recombinant human RNGTT protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Poids moléculaire: 70.9 kDa (620aa)

NCBI Accession: NP_003791

UniProt: O60942