DCT Protein (AA 24-472) (His tag)

N° du produit ABIN5854719

Détail du produit

Antigène: DCT (Dopachrome Tautomerase (DCT))

Type de proteíne: Recombinant

Attributs du protein: AA 24-472

Origine: Humain

Source: Baculovirus infected Insect Cells

Purification/Conjugué: Cette DCT protéine est marqué à la His tag.

Application: SDS-PAGE (SDS)

Séquence: QFPRVCMTVD SLVNKECCPR LGAESANVCG SQQGRGQCTE VRADTRPWSG PYILRNQDDR ELWPRKFFHR TCKCTGNFAG YNCGDCKFGW TGPNCERKKP PVIRQNIHSL SPQEREQFLG ALDLAKKRVH PDYVITTQHW LGLLGPNGTQ PQFANCSVYD FFVWLHYYSV RDTLLGPGRP YRAIDFSHQG PAFVTWHRYH LLCLERDLQR LIGNESFALP YWNFATGRNE CDVCTDQLFG AARPDDPTLI SRNSRFSSWE TVCDSLDDYN HLVTLCNGTY EGLLRRNQMG RNSMKLPTLK DIRDCLSLQK FDNPPFFQNS TFSFRNALEG FDKADGTLDS QVMSLHNLVH SFLNGTNALP HSAANDPIFV VLHSFTDAIF DEWMKRFNPP ADAWPQELAP IGHNRMYNMV PFFPPVTNEE LFLTSDQLGY SYAIDLPVSV EETPGWPTTH HHHHH

Pureté: > 90 % by SDS - PAGE

niveau d'endotoxine: < 1.0 EU per 1 microgram of protein (determined by LAL method)

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: Liquid. In Phosphate Buffered Saline (  pH 7.4) containing 10 % glycerol.

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Détail du antigène

Antigène: DCT (Dopachrome Tautomerase (DCT))

Autre désignation: DCT (DCT Produits)

Synonymes: TYRP2 Protein, RGD1564975 Protein, trp2 Protein, DT Protein, TRP-2 Protein, TRP2 Protein, Tyrp-2 Protein, Tyrp2 Protein, slaty Protein, slt Protein, zgc:109860 Protein, dopachrome tautomerase Protein, dopachrome tautomerase L homeolog Protein, DCT Protein, Dct Protein, dct Protein, dct.L Protein

Sujet: DCT, as known as L-dopachrome tautomerase isoform 1, is the tyrosinase family members. This protein plays a key role in the synthesis of the melanin pigment including tyrosinase (Tyr), tyrosinase-related protein 1 (Tyrp1). These are strongly implicated in eumelanin synthesis. The switch between the eumelanin and the pheomelanin pathways is thought to depend on the presence of cysteine. Thus, in the absence of cysteine, dopaquinone, the product of tyrosinase action, is transformed to cyclodopa (leucodopachrome) and then to dopachrome (and DOPA). Recombinant human DCT, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Poids moléculaire: 52.1kDa (455aa) 50-70kDa (SDS-PAGE under reducing conditions.)

NCBI Accession: NP_001913

UniProt: P40126