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DDT Protein (AA 21-248) (His tag)
DDT
Origine: Souris
Hôte: Escherichia coli (E. coli)
Recombinant
> 95 % by SDS - PAGE
SDS
N° du produit ABIN5855098
Détail du produit
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Antigène
Voir toutes DDT Protéines
DDT
(D-Dopachrome Tautomerase (DDT))
Type de proteíne
Recombinant
Attributs du protein
AA 21-248
Origine
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Souris
Source
Toutes les sources à travers DDT Protéines
Escherichia coli (E. coli)
Purification/Conjugué
Cette DDT protéine est marqué à la His tag.
Application
SDS-PAGE (SDS)
Séquence
MGSSHHHHHH SSGLVPRGSH MGSMPFVELE TNLPASRIPA GLENRLCAAT ATILDKPEDR VSVTIRPGMT LLMNKSTEPC AHLLVSSIGV VGTAEQNRTH SASFFKFLTE ELSLDQDRIV IRFFPLEAWQ IGKKGTVMTF L
Pureté
> 95 % by SDS - PAGE
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Information d'application
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Indications d'application
Optimal working dilution should be determined by the investigator.
Restrictions
For Research Use only
Stockage
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Format
Liquid
Concentration
1 mg/mL
Buffer
Liquid. In 20 mM Tris-Hcl buffer ( pH 8.0) containing 10 % glycerol
Stock
4 °C,-20 °C,-80 °C
Stockage commentaire
Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Détail du antigène
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Antigène
DDT
(D-Dopachrome Tautomerase (DDT))
Autre désignation
D-Dopachrome Decarboxylase (DDT Produits )
Synonymes
ddt-b Protein, zgc:86714 Protein, wu:fb49f11 Protein, C78655 Protein, DDT Protein, ddt Protein, ddt-a Protein, DDCT Protein, D-dopachrome tautomerase L homeolog Protein, D-dopachrome tautomerase Protein, D-dopachrome tautomerase S homeolog Protein, ddt.L Protein, DDT Protein, ddt Protein, Ddt Protein, ddt.S Protein
Sujet
Ddt, also known as D-dopachrome decarboxylase, is an enzyme that catayzes the tautomerization of D-dopachrome to give 5,6-dihyroxyindole(DHI). This protein belongs to the family of lyases, specifically the carboxy-lyases, which cleave carbon-carbon bonds. It shares a homologous amino acid sequence (33 % identical) with the macrophage migration inhibitory factor (MIF) and possesses similar tautomerase activity in addition, it may functions a proinflammatory cytokine. Recombinant mouse Ddt, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Poids moléculaire
15.5 kDa (141aa) confirmed by MALDI-TOF
NCBI Accession
NP_034157
UniProt
O35215
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