IL17D Protein (AA 35-169) (His tag,GST tag)

N° du produit ABIN6239768

Détail du produit

Antigène: IL17D (Interleukin 17D (IL17D))

Activité biologique: Active

Attributs du protein: AA 35-169

Origine: Humain

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette IL17D protéine est marqué à la His tag,GST tag.

Application: Activity Assay (AcA), Cell Culture (CC)

Attributs du produit: Tag location: N-terminal His and GST Tag

Pureté: > 90 %

Biological Activity Comment: IL17D (Interleukin-17D) belongs to the IL17 (interleukin 17) family, which includes six members (IL-17, IL-17B through IL-17F). IL17D is reported to induce the expression of IL6, CXCL8/IL8, and CSF2/GM-CSF from endothelial cells. IL17 family members share some similarities in AA sequence and structure, thus, a binding ELISA assay was constructed to detect the association of recombinant human IL17D with recombinant human IL17RB. Briefly, IL17D were diluted serially in PBS with 0.1%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL17RB-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-IL17D pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of IL17D with IL17RB was shown in Figure 1 and this effect was in a dose dependent manner The binding activity of IL17D with IL17RA.

Information d'application

Indications d'application: Isoelectric Point: 9.1

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.

Agent conservateur: Dithiothreitol (DTT), Other preservative, ProClin

Précaution d'utilisation: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Détail du antigène

Antigène: IL17D (Interleukin 17D (IL17D))

Abstract: IL17D Produits

Synonymes: IL-17D Protein, IL-22 Protein, IL-27 Protein, IL27 Protein, AI462269 Protein, Il27a Protein, il17-4 Protein, zgc:194257 Protein, zgc:194303 Protein, IL-27p28 Protein, Il30 Protein, p28 Protein, interleukin 17D Protein, interleukin 17d Protein, interleukin 27 Protein, IL17D Protein, Il17d Protein, il17d Protein, Il27 Protein

Sujet: Alternative Names: Interleukin-27

Poids moléculaire: 45kDa

UniProt: Q8TAD2

Pathways: Cellular Response to Molecule of Bacterial Origin