IL17RD Protein (AA 157-299) (His tag)

N° du produit ABIN6239809

Détail du produit

Antigène: IL17RD (Interleukin 17 Receptor D (IL17RD))

Activité biologique: Active

Attributs du protein: AA 157-299

Origine: Humain

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette IL17RD protéine est marqué à la His tag.

Application: Activity Assay (AcA), Cell Culture (CC)

Attributs du produit: Tag location: N-terminal His Tag

Pureté: > 90 %

Biological Activity Comment: IL17RD (interleukin-17 receptor D) is a membrane protein belonging to the IL17R protein family, acting as a component of the interleukin-17 receptor signaling complex. Knowing that the interaction between this protein and IL-17R does not require the interleukin, we have conducted a binding a binding ELISA assay to detect the interaction of recombinant human IL17RD with both recombinant human IL17RA and IL17. Briefly, IL17RD were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL17RA-coated or IL17-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-IL17RA pAb and anti-IL17 pAb separately, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution , wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of IL17RD with IL17RA and IL17 was shown in Figure 1 and Figure 2 respectively and this effect was in a dose dependent manner The binding activity of IL17RD with IL17RA. Figure 2. The binding activity of IL17RD with IL17.

Information d'application

Indications d'application: Isoelectric Point: 8.9

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.

Agent conservateur: Dithiothreitol (DTT), Other preservative, ProClin

Précaution d'utilisation: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Détail du antigène

Antigène: IL17RD (Interleukin 17 Receptor D (IL17RD))

Abstract: IL17RD Produits

Synonymes: IL17RD Protein, sef Protein, il-17rd Protein, il17rlm Protein, HH18 Protein, IL-17RD Protein, IL17RLM Protein, SEF Protein, 2810004A10Rik Protein, AI428510 Protein, Sef Protein, Sef-S Protein, RGD1565237 Protein, cb208 Protein, id:ibd2875 Protein, id:ibd5059 Protein, zgc:136328 Protein, interleukin 17 receptor D Protein, IL17RD Protein, il17rd Protein, Il17rd Protein

Sujet: Alternative Names: SEF, IL17RLM, IL-17RD, IL17R-D, IL17Rhom, Interleukin-17 receptor-like protein

Poids moléculaire: 20kDa

UniProt: Q8NFM7

Pathways: Toll-Like Receptors Cascades