ARG Protein (AA 1-323) (His tag)

N° du produit ABIN6239897

Détail du produit

Antigène: ARG (Arginase (ARG))

Activité biologique: Active

Attributs du protein: AA 1-323

Origine: Rat

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette ARG protéine est marqué à la His tag.

Application: Activity Assay (AcA), Cell Culture (CC)

Attributs du produit: Tag location: N-terminal His Tag

Pureté: > 97 %

Biological Activity Comment: Arginase (Arg) is an enzyme that catalyzes the degradation of arginine to produce urea and omithine, which is crucial in the urea cycle. In most mammals, two isozymes of this enzyme exist; the first, Arginase I, functions in the urea cycle, and is located primarily in the cytoplasm of the liver. The second isozyme, Arginase II, has been implicated in the regulation of the arginine/ornithine concentrations in the cell. Besides, Estrogen Receptor Alpha (ERa) has been identified as an interactor of, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat Arginase( Arg) and recombinant rat ERa. Briefly, Arg were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to ERa-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-Arg pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of of Arg and ERa was shown in Figure 1, and this effect was in a dose dependent manner The binding activity of Arg with ERa.

Information d'application

Indications d'application: Isoelectric Point: 6.7

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.

Agent conservateur: Dithiothreitol (DTT), Other preservative, ProClin

Précaution d'utilisation: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Détail du antigène

Antigène: ARG (Arginase (ARG))

Abstract: ARG Produits

Synonymes: CG18104 Protein, Dmel\\CG18104 Protein, EG:171D11.4 Protein, EG:65F1.3 Protein, SI:zC146F4.4 (novel protein with NUDIX domain) Protein, si:ch211-146f4.3 Protein, Tb08.26N11.490 Protein, rocF Protein, NV10213 Protein, argi1 Protein, arginase Protein, arginase 1 L homeolog Protein, arginase 1 Protein, Arginase-1 Protein, arg Protein, arg1.L Protein, arg1 Protein, ARGAH1 Protein, BP0538 Protein, rocF Protein, SAS2066 Protein, RR_RS05730 Protein, CND03500 Protein, CNG00550 Protein, Tb927.8.2020 Protein, PGTG_16455 Protein, LOC100123155 Protein, argi1 Protein

Sujet: Alternative Names: ARG1, Arginase I, Liver Arginase

Poids moléculaire: 37kDa

UniProt: P07824