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NSDHL Protein (AA 1-297) (His tag)
NSDHL
Origine: Humain
Hôte: Escherichia coli (E. coli)
Recombinant
> 80 % by SDS - PAGE
SDS
N° du produit ABIN6387344
Détail du produit
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Antigène
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NSDHL
(NAD(P) Dependent Steroid Dehydrogenase-Like (NSDHL))
Type de proteíne
Recombinant
Attributs du protein
AA 1-297
Origine
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Humain
Source
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Escherichia coli (E. coli)
Purification/Conjugué
Cette NSDHL protéine est marqué à la His tag.
Application
SDS-PAGE (SDS)
Séquence
MGSSHHHHHH SSGLVPRGSH MGSMEPAVSE PMRDQVARTH LTEDTPKVNA DIEKVNQNQA KRCTVIGGSG FLGQHMVEQL LARGYAVNVF DIQQGFDNPQ VRFFLGDLCS RQDLYPALKG VNTVFHCASP PPSSNNKELF YRVNYIGTKN VIETCKEAGV QKLILTSSAS VIFEGVDIKN GTEDLPYAMK PIDYYTETKI LQERAVLGAN DPEKNFLTTA IRPHGIFGPR DPQLVPILIE AARNGKMKFV IGNGKNLVDF TFVENVVHGH ILAAEQLSRD STLGGKAFHI TNDEPIPFWT FLSRILTGLN YEAPKYHIPY
Pureté
> 80 % by SDS - PAGE
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Information d'application
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Indications d'application
Optimal working dilution should be determined by the investigator.
Restrictions
For Research Use only
Stockage
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Format
Liquid
Concentration
0.25 mg/mL
Buffer
Liquid. 20 mM Tris-HCl buffer ( pH 8.0) containing 10 % glycerol 0.1M NaCl
Stock
4 °C,-20 °C,-80 °C
Stockage commentaire
Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Détail du antigène
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Antigène
NSDHL
(NAD(P) Dependent Steroid Dehydrogenase-Like (NSDHL))
Autre désignation
NSDHL (NSDHL Produits )
Synonymes
zgc:112474 Protein, H105E3 Protein, SDR31E1 Protein, XAP104 Protein, AI747449 Protein, Bpa Protein, Str Protein, NAD(P) dependent steroid dehydrogenase-like Protein, NAD(P) dependent steroid dehydrogenase-like L homeolog Protein, NSDHL Protein, nsdhl Protein, nsdhl.L Protein, Nsdhl Protein
Sujet
NSDHL, as known as Sterol-4-alpha-carboxylate 3-dehydrogenase, is involved in the production (synthesis) of cholesterol. During cholesterol synthesis, it participates in one of several steps that convert a molecule called lanosterol to cholesterol. Specifically, this enzyme removes a carbon atom and three hydrogen atoms (a methyl group) in the conversion of lanosterol to cholesterol. It is also an important component of cell membranes and myelin, the fatty covering that insulates nerve cells. Recombinant human NSDHL protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
Poids moléculaire
35.5kDa (320aa), confirmed by MALDI-TOF
NCBI Accession
NP_057006
UniProt
Q15738
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