IMPAD1 Protein (AA 34-359) (His tag)

N° du produit ABIN6387553

Détail du produit

Antigène: IMPAD1 (Inositol Monophosphatase Domain Containing 1 (IMPAD1))

Type de proteíne: Recombinant

Activité biologique: Active

Attributs du protein: AA 34-359

Origine: Humain

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette IMPAD1 protéine est marqué à la His tag.

Application: SDS-PAGE (SDS)

Séquence: MGSSHHHHHH SSGLVPRGSH MGSGRFSLFG LGGEPGGGAA GPAAAADGGT VDLREMLAVS VLAAVRGGDE VRRVRESNVL HEKSKGKTRE GAEDKMTSGD VLSNRKMFYL LKTAFPSVQI NTEEHVDAAD QEVILWDHKI PEDILKEVTT PKEVPAESVT VWIDPLDATQ EYTEDLRKYV TTMVCVAVNG KPMLGVIHKP FSEYTAWAMV DGGSNVKARS SYNEKTPRIV VSRSHSGMVK QVALQTFGNQ TTIIPAGGAG YKVLALLDVP DKSQEKADLY IHVTYIKKWD ICAGNAILKA LGGHMTTLSG EEISYTGSDG IEGGLLASIR MNHQALVRKL PDLEKTGHK

Pureté: > 90 % by SDS - PAGE

Biological Activity Comment: Specific acitivty > 3300 pmole/min/ug, its ability to dephosphorylate adenosine 3'5'-diphosphate sodium slat at pH 7.5, 25C.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

Bioactivity Validated

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: Liquid. In Phosphate Buffered Saline (  pH 7.4)

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Détail du antigène

Antigène: IMPAD1 (Inositol Monophosphatase Domain Containing 1 (IMPAD1))

Autre désignation: IMPAD1 (IMPAD1 Produits)

Synonymes: IMP 3 Protein, RGD1306455 Protein, gPAPP Protein, impa3 Protein, 1110001C20Rik Protein, AA408880 Protein, AI451589 Protein, AL022796 Protein, B230207P20 Protein, Jaws Protein, GPAPP Protein, IMP-3 Protein, IMPA3 Protein, IMPase 3 Protein, zgc:123256 Protein, inositol monophosphatase domain containing 1 Protein, inositol monophosphatase domain containing 1 S homeolog Protein, Impad1 Protein, impad1.S Protein, IMPAD1 Protein, impad1 Protein

Sujet: IMPAD1, also known as Inositol monophosphatase 3, is a member of the inositol monophosphatase family. IMPAD1 is localized to the Golgi apparatus and catalyzes the hydrolysis of phosphoadenosine phosphate (PAP) to adenosine monophosphate (AMP). Mutations in this gene are a cause of GRAPP type chondrodysplasia with joint dislocations, and a pseudogene of this gene is located on the long arm of chromosome 1. Recombinant human IMPAD1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

Poids moléculaire: 37.6kDa (349aa) confirmed by MALDI-TOF

NCBI Accession: NP_060283

UniProt: Q9NX62

Pathways: Glycosaminoglycan Metabolic Process